Yu Mengshan, Xiang Hanyue, Taledaohan Ayijiang, Fu Wenzhuo, Li Hongyu, Wang Yuji, Xiao Nao
Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
Core Facilities Center, Capital Medical University, Beijing 100069, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 15;329:125569. doi: 10.1016/j.saa.2024.125569. Epub 2024 Dec 9.
Hydroxyl radical is the most oxidizing type of reactive oxygen species (ROS), which is closely related to body health. In this work, a novel turn-on fluorescent probe BIJ-H for OH with high sensitivity was reported. BIJ-H was synthesized by a three-step reaction, and the pure product was obtained by only washing with poor solvents. In a neat aqueous solution containing BIJ-H, after adding OH, the probe was oxidized to BIJ, which produced fluorescence emission at 625 nm under the excitation with 550 nm. This sensing behavior can be achieved under physiological pH conditions. The detection limit of BIJ-H for OH was as low as 0.1379 μM. Thanks to the good analytical properties of BIJ-H, the probe has been applied to fluorescence imaging of OH in HepG2 cells and in acute liver injury induced by acetaminophen. The probe BIJ-H is expected to be used in the qualitative and quantitative detection of OH in physiological or pathological processes.
羟基自由基是活性氧物种（ROS）中氧化性最强的类型，它与身体健康密切相关。在这项工作中，报道了一种新型的对羟基具有高灵敏度的开启型荧光探针BIJ-H。BIJ-H通过三步反应合成，仅用不良溶剂洗涤即可获得纯产物。在含有BIJ-H的纯水溶液中，加入羟基后，探针被氧化为BIJ，在550nm激发下于625nm处产生荧光发射。这种传感行为可在生理pH条件下实现。BIJ-H对羟基的检测限低至0.1379μM。由于BIJ-H具有良好的分析性能，该探针已应用于HepG2细胞中羟基的荧光成像以及对乙酰氨基酚诱导的急性肝损伤中羟基的荧光成像。探针BIJ-H有望用于生理或病理过程中羟基的定性和定量检测。
