Bayir Hilal, Tacer Seyda, Yurdgulu Emir Enis, Albayrak Abdulmecit, Toktay Erdem, Bayir Yasin
Faculty of Fisheries, Department of Aquaculture, Ataturk University, Erzurum, 25240, Turkey.
Kocaali District Agriculture Directorate, Sakarya, 54800, Turkey.
J Mol Histol. 2024 Dec 14;56(1):41. doi: 10.1007/s10735-024-10320-3.
A peptic ulcer is a stomach lesion. It is generally caused by malnutrition, the use of anti-inflammatory medications, and an imbalance between mucosal defense systems. In fishes, the lubricous substance that called mucus secreted from the skin, prevents the entry of microorganisms that can enter the body through the skin. That mucus contains immune components such as antimicrobial peptides, lysozymes, lectins, proteases, and mucin. This study investigated the antiulcer activity of Rainbow Trout skin Mucus (RTM) in indomethacin induced ulcer model of rats and compared with famotidine as standard antiulcer drug. We administered 50, 100 and 200 mg/kg RTM dose on indomethacin-induced gastric ulcer model in rats and evaluated the numerical density of ulcer areas, histopathologic parameters and oxidative stress parameters (SOD, GSH, MDA) in the rat stomach. RTM was able to inhibit indomethacin-induced ulcer formation and exhibited a similar effect to 40 mg/kg dose of standard drug famotidine. 200 mg/kg dose of RTM had positive effects on oxidative stress biomarkers and histopathological results in the stomach tissue of rats. This is the first time we have fully demonstrated the gastroprotective effects of RTM as a waste product in rats. Analyses have shown that mucin, which has a positive regulatory effect on oxidative stress parameters, may be responsible for the gastroprotective effect.
消化性溃疡是一种胃部病变。它通常由营养不良、使用抗炎药物以及黏膜防御系统失衡引起。在鱼类中，从皮肤分泌的一种称为黏液的润滑物质可防止能通过皮肤进入体内的微生物侵入。该黏液含有免疫成分，如抗菌肽、溶菌酶、凝集素、蛋白酶和黏蛋白。本研究调查了虹鳟鱼皮肤黏液（RTM）在吲哚美辛诱导的大鼠溃疡模型中的抗溃疡活性，并与作为标准抗溃疡药物的法莫替丁进行比较。我们对吲哚美辛诱导的大鼠胃溃疡模型给予50、100和200mg/kg剂量的RTM，并评估大鼠胃中溃疡区域的数值密度、组织病理学参数和氧化应激参数（超氧化物歧化酶、谷胱甘肽、丙二醛）。RTM能够抑制吲哚美辛诱导的溃疡形成，且表现出与40mg/kg剂量的标准药物法莫替丁相似的效果。200mg/kg剂量的RTM对大鼠胃组织中的氧化应激生物标志物和组织病理学结果有积极影响。这是我们首次充分证明RTM作为大鼠体内一种废弃物的胃保护作用。分析表明，对氧化应激参数有正向调节作用的黏蛋白可能是胃保护作用的原因。
