Burnett P R, Wray D
Clin Immunol Immunopathol. 1985 Feb;34(2):197-204. doi: 10.1016/0090-1229(85)90024-8.
A radioisotope-release assay, utilizing 51Cr-labeled epithelial cells derived from non-keratinizing oral mucosa, was developed to investigate in vitro cytolytic reactions correlating with recurrent aphthous stomatitis (RAS). The cytolytic effects of sera and mononuclear leukocytes from patients in the early stage of ulceration were compared with those from matched RAS-negative control subjects. RAS sera induced significantly more cytolysis than did matched control sera. Heating the RAS or control sera for 30 min at 56 degrees C abrogated their cytotoxic activity. RAS mononuclear leukocytes, like their matched controls, showed no significant direct cytotoxicity. Heat-inactivated RAS or control sera acting in concert with RAS or control mononuclear leukocytes showed no consistent cytolytic effects. However, the heat-inactivated sera of some RAS patients, when combined with autologous mononuclear leukocytes, induced significantly more cytolysis than did either component acting alone. Thus heat-labile humoral factors and, in some cases, mononuclear leukocytes acting in concert with heat-stable serum factors are implicated in RAS-associated in vitro cytolytic reactions. These findings suggest that the effector mechanisms of such reactions include both complement-mediated and antibody-dependent cell-mediated cytotoxicity.
利用从非角化口腔黏膜衍生的51Cr标记上皮细胞开发了一种放射性同位素释放试验，以研究与复发性阿弗他口炎（RAS）相关的体外细胞溶解反应。将溃疡早期患者的血清和单核白细胞的细胞溶解作用与匹配的RAS阴性对照受试者的血清和单核白细胞的细胞溶解作用进行比较。RAS血清比匹配的对照血清诱导出明显更多的细胞溶解。将RAS或对照血清在56℃加热30分钟可消除其细胞毒性活性。RAS单核白细胞与其匹配的对照一样，未显示出明显的直接细胞毒性。热灭活的RAS或对照血清与RAS或对照单核白细胞协同作用未显示出一致的细胞溶解作用。然而，一些RAS患者的热灭活血清与自体单核白细胞联合使用时，诱导的细胞溶解比单独使用任何一种成分都明显更多。因此，热不稳定的体液因子以及在某些情况下与热稳定血清因子协同作用的单核白细胞与RAS相关的体外细胞溶解反应有关。这些发现表明，此类反应的效应机制包括补体介导的细胞毒性和抗体依赖性细胞介导的细胞毒性。
