Liu Yining, Kong Lingchao, Li Siyi, Nie Lingjun, Gao Junjie, Li Shuaitong, Pan Yuan, Liu Qingkun, Wang Zeying
College of Animal Science &Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Youth College of Political Science, Inner Mongolia Normal University, Hohhot 010051, China.
J Genet Eng Biotechnol. 2024 Dec;22(4):100440. doi: 10.1016/j.jgeb.2024.100440. Epub 2024 Nov 12.
Liaoning cashmere goat is an important livestock breed in the world. Its economic value is not only reflected in the production of high-quality cashmere, but also its meat production performance is increasingly attracting attention. In order to more comprehensively explore the economic traits of Liaoning cashmere goats, we mainly carry out research on increasing the body size and meat production performance of Liaoning cashmere goats. In the early stage, through multi-omics joint analysis of muscle tissues at different parts and different developmental stages, the important functional genes GH and IGF-1 for growth and development were determined. Then, genotyping of DNA in the experimental population and correlation analysis between SNP and production performance were carried out by PCR-seq. One SNP site G350A was detected in the GH gene. Only one genotype AG was identified in rams. In ewes, the GG genotype is the dominant genotype for body size and production performance, and the AG genotype is the dominant genotype for slaughter production performance. One SNP site C5464T was detected in the IGF-1 gene. In rams, the TT genotype is the dominant genotype for body size and slaughter production performance. In ewes, the CT genotype is the dominant genotype for body size and slaughter production performance. The dominant haplotype combination for body size and slaughter production performance of rams is AGTT. The dominant haplotype combination for body size production performance of ewes is GGCT, and the dominant haplotype combination for slaughter performance is AGCT. This study provides a theoretical basis for genetic improvement and breeding programs of Liaoning cashmere goats.
辽宁绒山羊是世界上重要的家畜品种。其经济价值不仅体现在优质羊绒的生产上，而且其产肉性能也越来越受到关注。为了更全面地探究辽宁绒山羊的经济性状，我们主要开展了提高辽宁绒山羊体尺和产肉性能的研究。前期，通过对不同部位、不同发育阶段的肌肉组织进行多组学联合分析，确定了生长发育的重要功能基因GH和IGF-1。然后，采用PCR-seq技术对试验群体的DNA进行基因分型，并分析SNP与生产性能之间的相关性。在GH基因中检测到一个SNP位点G350A。在公羊中只鉴定出一种基因型AG。在母羊中，GG基因型是体尺和生产性能的优势基因型，AG基因型是屠宰生产性能的优势基因型。在IGF-1基因中检测到一个SNP位点C5464T。在公羊中，TT基因型是体尺和屠宰生产性能的优势基因型。在母羊中，CT基因型是体尺和屠宰生产性能的优势基因型。公羊体尺和屠宰生产性能的优势单倍型组合是AGTT。母羊体尺生产性能的优势单倍型组合是GGCT，屠宰性能的优势单倍型组合是AGCT。本研究为辽宁绒山羊的遗传改良和育种计划提供了理论依据。
