Li Shuaitong, Kong Lingchao, Li Siyi, Liu Yining, Pan Yuan, Liu Qingkun, Hong Weihang, Ma Hua, Yuan Qingyu, Duan Ran, Zhan Qiying, Wang Zeying
College of Animal Science &Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
College of Animal Science &Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
J Genet Eng Biotechnol. 2024 Dec;22(4):100430. doi: 10.1016/j.jgeb.2024.100430. Epub 2024 Oct 19.
Liaoning cashmere goat (LCG) is characterized by the highest individual cashmere yield, but its cashmere fineness tends to be coarse. Therefore, our research primarily focuses on reducing cashmere fineness. Through lipidomics screening and identification, we identified the crucial functional genes FA2H and ELOVL3 associated with cashmere fineness. Subsequently, using PCR-seq, we conducted gene typing and SNP analysis on the experimental population DNA, In the FA2H gene, a SNP locus T42443G was detected in LCG buck, with the TT genotype showing advantageous traits in cashmere fineness, meat quality, and body size, while the TG genotype demonstrated advantages in slaughter performance,In LCG doe, the TG genotype shows advantageous traits in cashmere fineness, milk production, and meat quality, while the TT genotype exhibits advantages in slaughter performance, lambing, and body size. In the ELOVL3 gene, a SNP locus C2133A was identified in LCG buck, where the CC genotype was advantageous for cashmere fineness, Only CA genotype was found in slaughter and meat quality. Additionally, and the CA genotype showed superiority in body size. On LCG doe, The CC genotype was the advantageous genotype in terms of cashmere fineness, milk production, slaughter performance, and meat quality. The CA genotype was the advantageous genotype in terms of lambing and body size. The dominant genotypes identified to influence both doe cashmere fineness and slaughter performance were TT and CC. The identified dominant haplotype combination for cashmere production performance in LCG was CCTG. The dominant haplotype combination for doe slaughter performance was the CCTT haplotype combination. The dominant haplotype combination for buck slaughter performance was the CATG haplotype combination. Therefore, the TT genotype of the FA2H gene and the CC genotype of the ELOVL3 gene in LCG buck, and the TG genotype of the FA2H gene and the CC genotype of the ELOVL3 gene in doe can be used as molecular markers for assisted selection of cashmere fineness. CCTG haplotype combination was the superior haplotype combinations for cashmere production performance. To provide a theoretical basis for the breeding and expansion of fine-fiber type new strains of LCG.
辽宁绒山羊（LCG）以个体产绒量最高为特征，但其羊绒细度往往较粗。因此，我们的研究主要集中在降低羊绒细度上。通过脂质组学筛选和鉴定，我们确定了与羊绒细度相关的关键功能基因FA2H和ELOVL3。随后，利用PCR-seq对实验群体DNA进行基因分型和SNP分析，在FA2H基因中，在辽宁绒山羊公羊中检测到一个SNP位点T42443G，其中TT基因型在羊绒细度、肉质和体型方面表现出优势性状，而TG基因型在屠宰性能方面表现出优势；在辽宁绒山羊母羊中，TG基因型在羊绒细度、产奶量和肉质方面表现出优势性状，而TT基因型在屠宰性能、产羔和体型方面表现出优势。在ELOVL3基因中，在辽宁绒山羊公羊中鉴定出一个SNP位点C2133A，其中CC基因型有利于羊绒细度，在屠宰和肉质方面仅发现CA基因型，且CA基因型在体型方面表现出优势。在辽宁绒山羊母羊中，CC基因型在羊绒细度、产奶量、屠宰性能和肉质方面是优势基因型。CA基因型在产羔和体型方面是优势基因型。确定影响母羊羊绒细度和屠宰性能的显性基因型为TT和CC。确定的辽宁绒山羊产绒性能的显性单倍型组合为CCTG。母羊屠宰性能的显性单倍型组合为CCTT单倍型组合。公羊屠宰性能的显性单倍型组合为CATG单倍型组合。因此，辽宁绒山羊公羊中FA2H基因的TT基因型和ELOVL3基因的CC基因型，以及母羊中FA2H基因的TG基因型和ELOVL3基因的CC基因型可作为羊绒细度辅助选择的分子标记。CCTG单倍型组合是产绒性能的优良单倍型组合。为辽宁绒山羊细纤维型新品系的选育和扩繁提供理论依据。
