Lim P L, Rowley D
Int Arch Allergy Appl Immunol. 1985;76(1):30-6. doi: 10.1159/000233657.
Marked differences were seen in the oral absorption of Boivin antigen (BA) and flagellin (FLA) using antigens labelled by 3H-dinitrophenylation. Thus, after feeding 3H-DNP-FLA, a large proportion of the label was rapidly absorbed, concentrated and degraded by the liver so that no antigenic material could be recovered from the circulation. In contrast, the absorption of 3H-DNP-BA was low and slow; the absorbed material appeared stable and not readily taken up by the liver, so that 8-20% of the non-dialysable radioactivity found in the plasma was precipitable by specific antibodies. Such observations could not be made when 125I-labelled antigens were used: deiodination readily occurred in vivo, and there was re-utilization of the released label. On the other hand, using everted gut sacs, it was possible to recover 125I-labelled antigenic material after transport. A technique of measuring unlabelled antigen absorbed in the intact animal is also described, which detects antigen by combination with radioiodinated antibodies injected intravenously into the animal.
使用经³H -二硝基苯化标记的抗原，观察到波伊文抗原（BA）和鞭毛蛋白（FLA）的口服吸收存在显著差异。因此，喂食³H - DNP - FLA后，大部分标记物迅速被吸收、集中并被肝脏降解，以至于无法从循环中回收抗原性物质。相比之下，³H - DNP - BA的吸收缓慢且吸收率低；吸收的物质看起来稳定，且不易被肝脏摄取，因此血浆中发现的8 - 20%的非透析性放射性物质可被特异性抗体沉淀。当使用¹²⁵I标记的抗原时，则无法进行此类观察：体内很容易发生脱碘作用，且释放的标记物会被重新利用。另一方面，使用外翻肠囊时，运输后有可能回收¹²⁵I标记的抗原性物质。本文还描述了一种测量完整动物吸收的未标记抗原的技术，该技术通过与静脉注射到动物体内的放射性碘化抗体结合来检测抗原。
