Chen Jiabin, Li Shaobai, Kang Wenjun, Guan Shuyuan, Hong Zhihan, Liang Rongguang
Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA.
Biomed Opt Express. 2024 Nov 14;15(12):6834-6844. doi: 10.1364/BOE.543322. eCollection 2024 Dec 1.
The limited focal shift of conventional achromatic objectives constrains the maximum imaging depth of chromatic confocal microscopes. To address this, we designed a hyperchromatic confocal microscope using diffractive optical elements, which was fabricated by single-point diamond turning (SPDT). This design takes advantage of the small Abbe number of diffractive optical elements to introduce a significant longitudinal chromatic shift. The resulting chromatic confocal microscope achieved a maximum imaging depth of 750 µm and a lateral resolution of 0.78 µm across a wavelength range of 600-810 nm. The system's imaging capabilities were demonstrated by capturing detailed images of biological samples, including cucumber seed cavities, pig kidney, and human forearm skin. These results confirmed the microscope's effectiveness in visualizing key cellular structures, underscoring its potential for high-resolution biological imaging.
传统消色差物镜有限的焦移限制了色差共聚焦显微镜的最大成像深度。为了解决这个问题，我们设计了一种使用衍射光学元件的超色差共聚焦显微镜，该显微镜通过单点金刚石车削（SPDT）制造。这种设计利用衍射光学元件较小的阿贝数来引入显著的纵向色差。由此产生的色差共聚焦显微镜在600-810nm的波长范围内实现了750μm的最大成像深度和0.78μm的横向分辨率。通过拍摄包括黄瓜种子腔、猪肾和人体前臂皮肤在内的生物样本的详细图像，展示了该系统的成像能力。这些结果证实了该显微镜在可视化关键细胞结构方面的有效性，突出了其在高分辨率生物成像方面的潜力。
