Yang Xuefang, Wang Ye, Zhang Hejun, Qin Haoye, Wang Siyu, Tong Yihang, Zhou Keji, Sun Rui, Yue Shuhua, Chen Xun, Ding Shigang, Wang Pu
Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, China.
Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, China.
Biomed Opt Express. 2021 Dec 13;13(1):300-313. doi: 10.1364/BOE.442029. eCollection 2022 Jan 1.
Current endoscopy techniques have difficulties to provide both high resolution and large imaging depth, which significantly hinders the early diagnosis of gastric cancer. Here, we developed a label-free, large-depth, three-dimensional (3D) chromatic reflectance confocal endomicroscopy. In order to solve the problem of insufficient imaging depth of traditional chromatic confocal microscopy, a customized miniature objective lens both with large chromatic focal shift and correction for spherical aberration was used to focus light of different wavelengths at different depths of the sample simultaneously, and a fiber bundle containing 50000 single-mode cores was used to collect the confocal reflectance signal. To acquire detailed information along the axial direction at a faster speed, a high-speed multi-pixel spectrometer was used to realize simultaneous detection of multi-depth signals. Specifically, we have built up a label-free fiber-optic 3D chromatic reflectance confocal endomicroscopy, with 2.3 µm lateral resolution, imaging depth of 570 µm in 3D phantom and 220 µm in tissue, and 1.5 Hz 3D volumetric frame rate. We have demonstrated that the fiber-optic 3D chromatic confocal endomicroscopy can be used to image human gastric tissues ex vivo, and provide important morphological information for diagnosis without labeling. These results show the great potential of the fiber-optic 3D chromatic confocal endomicroscopy for gastric cancer diagnosis.
当前的内窥镜技术难以同时提供高分辨率和大成像深度，这严重阻碍了胃癌的早期诊断。在此，我们开发了一种无标记、大深度、三维（3D）彩色反射共聚焦内窥镜。为了解决传统彩色共聚焦显微镜成像深度不足的问题，我们使用了一种定制的微型物镜，其具有大的色差焦移并校正了球差，以便同时将不同波长的光聚焦在样品的不同深度处，并且使用了包含50000个单模芯的光纤束来收集共聚焦反射信号。为了更快地获取沿轴向的详细信息，使用了高速多像素光谱仪来实现多深度信号的同时检测。具体而言，我们构建了一种无标记的光纤3D彩色反射共聚焦内窥镜，其横向分辨率为2.3 µm，在3D模型中的成像深度为570 µm，在组织中的成像深度为220 µm，3D体积帧率为1.5 Hz。我们已经证明，光纤3D彩色共聚焦内窥镜可用于离体成像人体胃组织，并在不标记的情况下为诊断提供重要的形态学信息。这些结果表明光纤3D彩色共聚焦内窥镜在胃癌诊断方面具有巨大潜力。
