Seki David, Kirkegaard Rasmus, Osvatic Jay, Hausmann Bela, Séneca Joana, Pjevac Petra, Berger Angelika, J Hall Lindsay, Wisgrill Lukas, Berry David
Centre for Microbiology and Environmental Systems Science, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, Vienna, Austria.
Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, Vienna, Austria.
Gut Microbes. 2024 Jan-Dec;16(1):2410479. doi: 10.1080/19490976.2024.2410479. Epub 2024 Oct 7.
Severe brain damage is common among premature infants, and the gut microbiota has been implicated in its pathology. Although the order of colonizing bacteria is well described, the mechanisms underlying aberrant assembly of the gut microbiota remain elusive. Here, we employed long-read nanopore sequencing to assess abundances of microbial species and their functional genomic potential in stool samples from a cohort of 30 extremely premature infants. We identify several key microbial traits significantly associated with severe brain damage, such as the genomic potential for nitrate respiration and iron scavenging. Members of the were prevalent across the cohort and displayed a versatile metabolic potential, including pathogenic and nonpathogenic traits. Predominance of and were associated with an overall loss of genomic functional redundancy as well as poor neurophysiological outcome. These findings reveal microbial traits that may be involved in exacerbating brain injury in extremely premature infants and provide suitable targets for therapeutic interventions.
严重脑损伤在早产儿中很常见，肠道微生物群被认为与该病的病理过程有关。尽管定殖细菌的顺序已得到充分描述，但肠道微生物群异常组装的潜在机制仍不清楚。在这里，我们采用长读长纳米孔测序技术，评估了30名极早产儿队列粪便样本中微生物种类的丰度及其功能基因组潜力。我们确定了几个与严重脑损伤显著相关的关键微生物特征，如硝酸盐呼吸和铁清除的基因组潜力。该队列中 的成员普遍存在，并表现出多种代谢潜力，包括致病和非致病特征。 和 的优势与基因组功能冗余的整体丧失以及不良的神经生理结果有关。这些发现揭示了可能参与加重极早产儿脑损伤的微生物特征，并为治疗干预提供了合适的靶点。
