Department of Food Chemistry and Toxicology, University of Vienna, 1090 Vienna, Austria.
Center for Microbiology and Environmental Systems Science, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, 1090 Vienna, Austria; Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, 1090 Vienna, Austria.
Cell Rep Med. 2024 Apr 16;5(4):101480. doi: 10.1016/j.xcrm.2024.101480. Epub 2024 Mar 22.
The gut microbiome is associated with pathological neurophysiological evolvement in extremely premature infants suffering from brain injury. The exact underlying mechanism and its associated metabolic signatures in infants are not fully understood. To decipher metabolite profiles linked to neonatal brain injury, we investigate the fecal and plasma metabolome of samples obtained from a cohort of 51 extremely premature infants at several time points, using liquid chromatography (LC)-high-resolution mass spectrometry (MS)-based untargeted metabolomics and LC-MS/MS-based targeted analysis for investigating bile acids and amidated bile acid conjugates. The data are integrated with 16S rRNA gene amplicon gut microbiome profiles as well as patient cytokine, growth factor, and T cell profiles. We find an early onset of differentiation in neuroactive metabolites between infants with and without brain injury. We detect several bacterially derived bile acid amino acid conjugates in plasma and feces. These results provide insights into the early-life metabolome of extremely premature infants.
肠道微生物群与脑损伤的极早产儿的病理性神经生理演变有关。在婴儿中，其确切的潜在机制及其相关的代谢特征尚不完全清楚。为了解析与新生儿脑损伤相关的代谢物谱，我们使用基于液相色谱（LC）-高分辨率质谱（MS）的非靶向代谢组学和基于 LC-MS/MS 的靶向分析，对来自 51 名极早产儿队列的样本的粪便和血浆代谢组进行了研究，以调查胆汁酸和酰胺化胆汁酸结合物。将这些数据与 16S rRNA 基因扩增子肠道微生物组图谱以及患者细胞因子、生长因子和 T 细胞图谱进行了整合。我们发现有和无脑损伤的婴儿之间神经活性代谢物的早期分化。我们在血浆和粪便中检测到几种细菌衍生的胆汁酸氨基酸缀合物。这些结果为极早产儿的早期生命代谢组学提供了深入的了解。
