Kim Elizabeth, Nealon Nora Jean, Murray Katherine A, Jardine Cydney, Magnuson Roberta, Rao Sangeeta
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Department of Diagnostic Medicine & Pathobiology, Shreiber School of Veterinary Medicine, Rowan University, Mullica Hill, NJ 08062, USA.
Animals (Basel). 2024 Dec 2;14(23):3483. doi: 10.3390/ani14233483.
This study investigates antimicrobial-resistant (AMR) Typhimurium in poultry, focusing on how class I integrons contribute to AMR and virulence. Using whole genome sequencing, researchers analyzed 26 Typhimurium isolates from U.S. poultry, finding that three isolates contained integrons (1000 base pairs each). These integron-positive isolates exhibited significantly higher resistance to beta-lactams, phenicols, and tetracyclines compared to integron-free isolates ( = 0.004, 0.009, and 0.02, respectively) and harbored genes like , , and , which are linked to extended-spectrum beta-lactamase resistance. Most AMR gene classes (64%) were chromosome-based, with integron-positive isolates showing a broader array of resistance genes, including and . Integron-bearing isolates had higher occurrences of bacteriocin genes and specific AMR genes like aminoglycoside and beta-lactam resistance genes, while integron-free isolates had more fimbrial and pilus genes. The presence of integrons may trend with increased AMR genes and virulence factors, highlighting the role of integron screening in enhancing AMR surveillance and reducing the need for high-priority antimicrobial treatments in poultry. These findings could support better AMR stewardship practices in poultry production, potentially lowering infection risks in humans and livestock.
本研究调查了家禽中的耐抗菌药（AMR）鼠伤寒沙门氏菌，重点关注I类整合子如何促进抗菌耐药性和毒力。研究人员使用全基因组测序分析了来自美国家禽的26株鼠伤寒沙门氏菌分离株，发现其中三株含有整合子（每株1000个碱基对）。与无整合子的分离株相比，这些整合子阳性分离株对β-内酰胺类、氯霉素和四环素表现出显著更高的耐药性（分别为 = 0.004、0.009和0.02），并携带了如 、 和 等与超广谱β-内酰胺酶耐药性相关的基因。大多数AMR基因类别（64%）基于染色体，整合子阳性分离株显示出更广泛的耐药基因阵列，包括 和 。携带整合子的分离株中细菌素基因和特定AMR基因（如氨基糖苷和β-内酰胺耐药基因）的出现频率更高，而无整合子的分离株中菌毛和菌毛基因更多。整合子的存在可能与AMR基因和毒力因子的增加有关，突出了整合子筛查在加强AMR监测和减少家禽中高优先级抗菌治疗需求方面的作用。这些发现可为家禽生产中更好的AMR管理实践提供支持，有可能降低人类和牲畜的感染风险。
