Huang Ningxin, Wang Zhengxiao, Xiao Xiao, Gai Te'er, Zhao Dongyue, Liu Lu, Wu Wei
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Dongying 257500, China.
Foods. 2024 Nov 26;13(23):3811. doi: 10.3390/foods13233811.
Utilizing (), this study constructed a dual-chamber microbial electrosynthesis system, based on microbial electrolysis cells, that was capable of producing lycopene. Cultivation within the electrosynthesis chamber yielded a lycopene concentration of 282.3722 mg/L when the optical density (OD) reached 0.6, which was four times greater than that produced by original strains. The mutant strain showed significantly higher levels of extracted riboflavin compared to the wild-type strain, and the riboflavin content of the mutant strain was 61.081 mg/L, which was more than 10 times that of the original strain. Furthermore, sequencing and analyses were performed on the mutant strains observed during the experiment. The results indicated differences in antibiotic resistance genes, carbohydrate metabolism-related genes, and the frequencies of functional genes between the mutant and original strains. The mutant strain displayed potential advantages in specific antibiotic resistance and carbohydrate degradation capabilities, likely attributable to its adaptation to electrogenic growth conditions. Moreover, the mutant strain demonstrated an enrichment of gene frequencies associated with transcriptional regulation, signal transduction, and amino acid metabolism, suggesting a complex genetic adaptation to electrogenic environments. This study presents a novel approach for the efficient and energy-conserving production of lycopene while also providing deeper insights into the genetic basis of electro-resistance genes.
本研究利用（）构建了基于微生物电解池的双室微生物电合成系统，该系统能够生产番茄红素。当光密度（OD）达到0.6时，在电合成室内培养得到的番茄红素浓度为282.3722mg/L，比原始菌株产生的浓度高出四倍。与野生型菌株相比，突变菌株提取的核黄素水平显著更高，突变菌株的核黄素含量为61.081mg/L，是原始菌株的10倍多。此外，对实验过程中观察到的突变菌株进行了测序和分析。结果表明，突变菌株与原始菌株在抗生素抗性基因、碳水化合物代谢相关基因以及功能基因频率方面存在差异。突变菌株在特定抗生素抗性和碳水化合物降解能力方面表现出潜在优势，这可能归因于其对产电生长条件的适应性。此外，突变菌株显示出与转录调控、信号转导和氨基酸代谢相关的基因频率富集，表明其对产电环境具有复杂的遗传适应性。本研究提出了一种高效节能生产番茄红素的新方法，同时也为抗电基因的遗传基础提供了更深入的见解。
