Xu Yiqun, Jiang Liling, Gao Jia, Zhang Wei, Zhang Meijun, Liu Changlai, Jia Juqing
College of Agriculture, Shanxi Agricultural University, Jinzhong 030810, China.
Academy of Agricultural and Forestry Sciences, Qinghai University National Duplicate Genebank for Crops, Xining 810016, China.
Plants (Basel). 2024 Nov 26;13(23):3317. doi: 10.3390/plants13233317.
Common oat ( L.) is one of the important minor grain crops in China, and drought stress severely affects its yield and quality. To investigate the drought resistance characteristics of oat seedlings, this study used Baiyan 2, an oat cultivar at the three-leaf stage, as the experimental material. Drought stress was simulated using polyethylene glycol (PEG) to treat the seedlings. The photosynthetic parameters and physicochemical indices of the treatment groups at 6 h and 12 h were measured and compared with the control group at 0 h. The results showed that drought stress did not significantly change chlorophyll content, but it significantly reduced net photosynthetic rate and other photosynthetic parameters while significantly increasing proline content. Transcriptome analysis was conducted using seedlings from both the control and treatment groups, comparing the two treatment groups with the control group using Tbtool software (v2.136). This analysis identified 344 differentially expressed genes. Enrichment analysis of these differentially expressed genes revealed significant enrichment in physiological pathways such as photosynthesis and ion transport. Ten differentially expressed genes related to the physiological process of photosynthetic carbon assimilation were identified, all of which were downregulated. Additionally, seven differentially expressed genes were related to ion transport. Through gene co-expression analysis combined with promoter region structure analysis, 11 transcription factors (from , , ) were found to regulate the expression of 10 genes related to photosynthetic carbon assimilation. Additionally, five transcription factors regulate the expression of two malate transporter protein-related genes (from , , , etc.), five transcription factors regulate the expression of two metal ion transporter protein-related genes (from , , ), five transcription factors regulate the expression of two chloride channel protein-related genes (from , , ), and two transcription factors regulate the expression of one Annexin-related gene (from , ). This study provides a theoretical foundation for further research on the molecular regulation of guard cells and offers a molecular basis for enhancing drought resistance in oats.
普通燕麦（L.）是中国重要的小杂粮作物之一，干旱胁迫严重影响其产量和品质。为了研究燕麦幼苗的抗旱特性，本研究以三叶期燕麦品种白燕2号为试验材料。采用聚乙二醇（PEG）模拟干旱胁迫处理幼苗。测定处理组在6小时和12小时时的光合参数和理化指标，并与0小时的对照组进行比较。结果表明，干旱胁迫对叶绿素含量影响不显著，但显著降低了净光合速率等光合参数，同时显著提高了脯氨酸含量。对对照组和处理组的幼苗进行转录组分析，使用Tbtool软件（v2.136）将两个处理组与对照组进行比较。该分析鉴定出344个差异表达基因。对这些差异表达基因的富集分析表明，它们在光合作用和离子运输等生理途径中显著富集。鉴定出10个与光合碳同化生理过程相关的差异表达基因，均下调表达。此外，有7个差异表达基因与离子运输有关。通过基因共表达分析结合启动子区域结构分析，发现11个转录因子（来自……）调控10个与光合碳同化相关基因的表达。此外，5个转录因子调控2个与苹果酸转运蛋白相关基因（来自……等）的表达，5个转录因子调控2个与金属离子转运蛋白相关基因（来自……）的表达，5个转录因子调控2个与氯离子通道蛋白相关基因（来自……）的表达，2个转录因子调控1个与膜联蛋白相关基因（来自……）的表达。本研究为进一步研究保卫细胞的分子调控提供了理论基础，也为提高燕麦抗旱性提供了分子依据。
