Kim Jeong-Hyeon, Lee Ji Young, Lee Juri, Hillman Prima F, Lee Jihye, Choi Byeongchan, Paik Man-Jeong, Lee Songyi, Nam Sang-Jip
Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.
Institute of Sustainable Earth and Environmental Dynamics (SEED), Pukyong National University, 365 Sinseon-ro, Nam-gu, Busan 48547, Republic of Korea.
Molecules. 2024 Nov 23;29(23):5539. doi: 10.3390/molecules29235539.
Three new depsipeptides, homiamides A-C (-), were isolated from a marine sediment-derived strain of sp., ROA-065. The planar structures of homiamides A-C (-) were elucidated using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopic data. The absolute configurations of - were deduced from the application of the Marfey's method and GC-MS analysis after formation of the -trifluoroacetylated ()-(+)-methyl-2-butyl ester derivatives of amino acids. Compounds - exhibited weak anti-bacterial activities against both Gram-positive bacteria and Gram-negative bacteria, with compound showing MIC values of 32-64 μg/mL. In antifouling assays, compounds and displayed moderate activity against KCTC 3063, while compound exhibited weak activity against all tested bacteria.
从海洋沉积物来源的菌株ROA-065中分离出三种新的缩肽，即高酰胺A-C（-）。利用质谱（MS）和核磁共振（NMR）光谱数据阐明了高酰胺A-C（-）的平面结构。通过应用马尔菲方法并在氨基酸的三氟乙酰化（）-（+）-甲基-2-丁酯衍生物形成后进行气相色谱-质谱（GC-MS）分析，推断出-的绝对构型。化合物-对革兰氏阳性菌和革兰氏阴性菌均表现出较弱的抗菌活性，化合物的最低抑菌浓度（MIC）值为32-64μg/mL。在防污试验中，化合物和对KCTC 3063表现出中等活性，而化合物对所有测试细菌均表现出较弱活性。
