Jin Feng, Guan Zhengrong, Zhang Jiahao, Qu Zhigang, Ling Shengjie, Cao Leitao, Ren Jing, Peng Ruoxuan
Shengzhou Mulsun Biotech Co., Ltd., 99 Jincan Road, Shengzhou 312499, China.
School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, China.
Polymers (Basel). 2024 Nov 25;16(23):3281. doi: 10.3390/polym16233281.
Seed coating technology is vital in agriculture, enhancing seed protection and growth. However, conventional coatings often include chemical fungicides that pose environmental risks, highlighting the need for sustainable alternatives. This study explores silk fibroin (SF), a natural biopolymer with excellent film-forming properties, as a potential seed coating agent, addressing its antimicrobial limitations by combining it with the commercial agent CRUISER and the antimicrobial peptide Nisin. Experimental methods included solution stability analysis, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and growth assessments of wheat seeds. Findings reveal that silk fibroin-CRUISER (SC) composites form stable β-sheet structures, enhancing the coating's mechanical strength. SF-based coatings improved seedling emergence rates (up to 1.65-fold), plant height (up to 1.05-fold), and root growth (up to 1.2-fold), especially under cold stress. The addition of Nisin further significantly boosted the antibacterial properties, providing sustained pathogen inhibition ( < 0.01). Identifying the optimal concentration of SF was essential for achieving a balance between protection and breathability, a key factor for industrial application. This research provides valuable insights into the development of eco-friendly seed coatings, presenting a viable and sustainable alternative to traditional chemical-based options in agricultural practices.
种子包衣技术在农业中至关重要，可增强种子保护和生长。然而，传统包衣通常含有对环境有风险的化学杀菌剂，这凸显了对可持续替代方案的需求。本研究探索了丝素蛋白（SF），一种具有优异成膜性能的天然生物聚合物，作为潜在的种子包衣剂，并通过将其与商业制剂CRUISER和抗菌肽Nisin结合来解决其抗菌局限性。实验方法包括溶液稳定性分析、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）以及小麦种子的生长评估。研究结果表明，丝素蛋白 - CRUISER（SC）复合材料形成稳定的β - 片层结构，增强了包衣的机械强度。基于SF的包衣提高了种子出苗率（高达1.65倍）、株高（高达1.05倍）和根系生长（高达1.2倍），尤其是在冷胁迫下。添加Nisin进一步显著增强了抗菌性能，提供了持续的病原体抑制作用（<0.01）。确定SF的最佳浓度对于在保护和透气性之间取得平衡至关重要，这是工业应用的关键因素。本研究为环保型种子包衣的开发提供了有价值的见解，为农业实践中的传统化学基选项提供了可行且可持续的替代方案。
