• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

年龄相关性、糖尿病性和玻璃体切除术后白内障中糖脂代谢途径的蛋白质组学变化

Proteomic Changes of Glycolipid Pathways in Age-Related, Diabetic, and Post-Vitrectomy Cataracts.

作者信息

Karakosta Christina, Samiotaki Martina, Panayotou George, Papaconstantinou Dimitrios, Moschos Marilita M

机构信息

School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece.

Biomedical Sciences Research Center "Alexander Fleming", 16672 Vari, Greece.

出版信息

J Clin Med. 2024 Nov 30;13(23):7287. doi: 10.3390/jcm13237287.

DOI:10.3390/jcm13237287
PMID:39685745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11642120/
Abstract

: Alterations in glycolipid and glycosphingolipid pathways lead to compromised cell membranes and may be involved in cataract formation. However, the exact role of glycolipids in lens opacification is not completely understood. The aim of the current study is to investigate proteome complexity and the role of glycolipid and glycosphingolipid pathways in cataract formation. : The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from eleven participants with diabetic cataract (DC), twelve participants with age-related cataract (ARC), and seven participants with post-vitrectomy cataract (PVC). Liquid chromatography-mass spectrometry with data-independent acquisition (DIA) was used for the identification and quantification of proteins. : The results of this study revealed that the main significantly differentially expressed pathways in the ARC group compared to the DC and PVC groups in phaco cassette samples included the glycolipid metabolic, glycosphingolipid biosynthetic, and glycosphingolipid metabolic processes, with GLA being among the most significant proteins in the ARC group. Similarly, in the anterior capsule samples, the main significantly differentially expressed pathways in the ARC group compared to the DC and PVC groups were the glycolipid metabolic, glycosphingolipid biosynthetic, and glycosphingolipid metabolic processes, with ST3GAL5 being among the most significant proteins in the ARC group. : Glycolipid and glycosphingolipid metabolic processes may be involved in cataract formation. ST3GAL5 may modify the cell-to-cell interaction induced by cell surface sugar chains, leading to the formation and progression of cataract. GLA, associated with the breakdown of glycolipids, may lead to cataract formation when a certain threshold is surpassed, secondary to increased glycolipid metabolism.

摘要

糖脂和糖鞘脂途径的改变会导致细胞膜受损，可能与白内障的形成有关。然而，糖脂在晶状体混浊中的具体作用尚未完全明确。本研究的目的是探讨蛋白质组复杂性以及糖脂和糖鞘脂途径在白内障形成中的作用。：在白内障手术过程中，收集了11例糖尿病性白内障（DC）患者、12例年龄相关性白内障（ARC）患者和7例玻璃体切除术后白内障（PVC）患者的前囊膜和超声乳化（phaco）盒内容物。采用数据非依赖采集（DIA）的液相色谱-质谱法对蛋白质进行鉴定和定量。：本研究结果显示，与DC组和PVC组相比，ARC组在phaco盒样本中主要显著差异表达的途径包括糖脂代谢、糖鞘脂生物合成和糖鞘脂代谢过程，其中GLA是ARC组中最显著的蛋白质之一。同样，在前囊膜样本中，与DC组和PVC组相比，ARC组主要显著差异表达的途径也是糖脂代谢、糖鞘脂生物合成和糖鞘脂代谢过程，其中ST3GAL5是ARC组中最显著的蛋白质之一。：糖脂和糖鞘脂代谢过程可能参与白内障的形成。ST3GAL5可能会改变细胞表面糖链诱导的细胞间相互作用，导致白内障的形成和进展。与糖脂分解相关的GLA在糖脂代谢增加导致超过一定阈值时，可能会导致白内障的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/134394d3d989/jcm-13-07287-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/2f0cc8311dd2/jcm-13-07287-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/48537a8c2aec/jcm-13-07287-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/08920a048128/jcm-13-07287-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/091899ce2b4b/jcm-13-07287-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/7e52ff5ecca5/jcm-13-07287-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/363b77e6d3bd/jcm-13-07287-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/5ed0fa380347/jcm-13-07287-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/f319c666f393/jcm-13-07287-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/130a922de263/jcm-13-07287-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/2a6d27e36c22/jcm-13-07287-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/134394d3d989/jcm-13-07287-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/2f0cc8311dd2/jcm-13-07287-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/48537a8c2aec/jcm-13-07287-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/08920a048128/jcm-13-07287-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/091899ce2b4b/jcm-13-07287-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/7e52ff5ecca5/jcm-13-07287-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/363b77e6d3bd/jcm-13-07287-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/5ed0fa380347/jcm-13-07287-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/f319c666f393/jcm-13-07287-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/130a922de263/jcm-13-07287-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/2a6d27e36c22/jcm-13-07287-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb2/11642120/134394d3d989/jcm-13-07287-g011.jpg

相似文献

1
Proteomic Changes of Glycolipid Pathways in Age-Related, Diabetic, and Post-Vitrectomy Cataracts.年龄相关性、糖尿病性和玻璃体切除术后白内障中糖脂代谢途径的蛋白质组学变化
J Clin Med. 2024 Nov 30;13(23):7287. doi: 10.3390/jcm13237287.
2
Differential Signaling Pathways Identified in Aqueous Humor, Anterior Capsule, and Crystalline Lens of Age-Related, Diabetic, and Post-Vitrectomy Cataract.在年龄相关性、糖尿病性和玻璃体切除术后白内障的房水、前囊膜和晶状体中鉴定出的差异信号通路。
Proteomes. 2025 Feb 3;13(1):7. doi: 10.3390/proteomes13010007.
3
Role of actin-binding proteins in cataract formation.肌动蛋白结合蛋白在白内障形成中的作用。
Cytoskeleton (Hoboken). 2025 Mar;82(3):98-110. doi: 10.1002/cm.21889. Epub 2024 Jul 3.
4
Senile cataract-related accumulation of Lewis(x) glycolipid in human lens.老年人白内障相关的Lewis(x)糖脂在人晶状体中的蓄积。
J Biol Chem. 1992 Apr 5;267(10):6467-70.
5
Application of prechop technique in phacoemulsification for cataract patients with highly liquefied vitreous: a retrospective study.超声乳化前劈核技术在高度液化玻璃体白内障患者中的应用：一项回顾性研究。
BMC Ophthalmol. 2022 Apr 14;22(1):167. doi: 10.1186/s12886-022-02392-0.
6
Effect of femtosecond laser-assisted cataract surgery for cataracts after pars plana vitrectomy: a prospective randomized controlled study.飞秒激光辅助白内障手术治疗玻璃体切割术后白内障的效果：一项前瞻性随机对照研究。
BMC Ophthalmol. 2025 Feb 17;25(1):79. doi: 10.1186/s12886-025-03871-w.
7
Glycosphingolipid patterns of the epithelial and non-epithelial compartments of rat large intestine.大鼠大肠上皮和非上皮区室的糖鞘脂模式
Biochim Biophys Acta. 1984 Mar 7;792(3):281-92. doi: 10.1016/0005-2760(84)90195-4.
8
A lipidomic study on the lens epithelial cells of patients with age related cataracts.年龄相关性白内障患者晶状体上皮细胞的脂质组学研究。
PeerJ. 2024 Sep 6;12:e17998. doi: 10.7717/peerj.17998. eCollection 2024.
9
RNA-Seq Study of Human Lens Epithelial Cells: Differentially Expressed Genes and Pathways in Steroid, Uveitic, Post-Vitrectomy, and Senile Cataracts.人晶状体上皮细胞的RNA测序研究：类固醇性、葡萄膜炎性、玻璃体切除术后及老年性白内障中的差异表达基因和通路
Invest Ophthalmol Vis Sci. 2025 May 1;66(5):4. doi: 10.1167/iovs.66.5.4.
10
Retrospective analysis of risk factors for unplanned anterior vitrectomy during planned cataract ultrasonic phacoemulsification in Chinese adult patients.中国成年患者计划性白内障超声乳化术中非计划性前部玻璃体切割术危险因素的回顾性分析
BMC Ophthalmol. 2025 Apr 9;25(1):186. doi: 10.1186/s12886-025-04012-z.

引用本文的文献

1
Differential Signaling Pathways Identified in Aqueous Humor, Anterior Capsule, and Crystalline Lens of Age-Related, Diabetic, and Post-Vitrectomy Cataract.在年龄相关性、糖尿病性和玻璃体切除术后白内障的房水、前囊膜和晶状体中鉴定出的差异信号通路。
Proteomes. 2025 Feb 3;13(1):7. doi: 10.3390/proteomes13010007.

本文引用的文献

1
Role of actin-binding proteins in cataract formation.肌动蛋白结合蛋白在白内障形成中的作用。
Cytoskeleton (Hoboken). 2025 Mar;82(3):98-110. doi: 10.1002/cm.21889. Epub 2024 Jul 3.
2
Lens Cytoskeleton: An Update on the Etiopathogenesis of Human Cataracts.晶状体细胞骨架：人类白内障病因发病机制的最新进展
Cureus. 2024 Mar 23;16(3):e56793. doi: 10.7759/cureus.56793. eCollection 2024 Mar.
3
The ProteomeXchange consortium at 10 years: 2023 update.蛋白质组交换联盟成立十周年：2023 年更新。
Nucleic Acids Res. 2023 Jan 6;51(D1):D1539-D1548. doi: 10.1093/nar/gkac1040.
4
Changes in the Lipid Profile of Aqueous Humor From Diabetic Cataract Patients.糖尿病性白内障患者房水中脂类谱的变化。
Transl Vis Sci Technol. 2022 Nov 1;11(11):5. doi: 10.1167/tvst.11.11.5.
5
Alterations in Lysosome Homeostasis in Lipid-Related Disorders: Impact on Metabolic Tissues and Immune Cells.脂质相关疾病中溶酶体稳态的改变：对代谢组织和免疫细胞的影响
Front Cell Dev Biol. 2021 Dec 10;9:790568. doi: 10.3389/fcell.2021.790568. eCollection 2021.
6
Solvent-resistant microfluidic paper-based analytical device/spray mass spectrometry for quantitative analysis of C -ceramide biomarker.耐溶剂微流控纸基分析装置/喷雾质谱法用于 C-神经酰胺生物标志物的定量分析。
J Mass Spectrom. 2021 Apr;56(4):e4611. doi: 10.1002/jms.4611. Epub 2020 Aug 12.
7
Lipid conformational order and the etiology of cataract and dry eye.脂质构象有序性与白内障和干眼症的病因
J Lipid Res. 2021;62:100039. doi: 10.1194/jlr.TR120000874. Epub 2021 Feb 6.
8
Gangliosides: The Double-Edge Sword of Neuro-Ectodermal Derived Tumors.神经外胚层来源肿瘤的双刃剑：神经节苷脂
Biomolecules. 2019 Jul 27;9(8):311. doi: 10.3390/biom9080311.
9
Advances in determining signaling mechanisms of ceramide and role in disease.神经酰胺信号机制的研究进展及其在疾病中的作用
J Lipid Res. 2019 May;60(5):913-918. doi: 10.1194/jlr.S092874. Epub 2019 Mar 7.
10
Sphingolipids as Regulators of Autophagy and Endocytic Trafficking.鞘脂作为自噬和内吞运输的调节剂。
Adv Cancer Res. 2018;140:27-60. doi: 10.1016/bs.acr.2018.04.008. Epub 2018 May 22.