Pandey Vaibhav, Gupta Amit, Choudhary Indra Singh, Imran Mohammad, Mudavath Shyam Lal, Kar Amrita Ghosh, Nandan Ruchira
Department of Pediatric Surgery, IMS-BHU, Varanasi, Uttar Pradesh, India.
Department of Pediatric Surgery, AIIMS, Bhopal, Madhya Pradesh, India.
J Indian Assoc Pediatr Surg. 2024 Nov-Dec;29(6):612-616. doi: 10.4103/jiaps.jiaps_99_24. Epub 2024 Nov 5.
The use of silver nanoparticles (AgNPs) in biomedicine has emerged in a big way owing to its antibacterial and anti-inflammatory properties. We hypothesize that combining the AgNPs with antibiotics for coating sutures will enhance the antibacterial property of sutures with the added advantage of the immunomodulatory effect of AgNPs on tissue healing.
Polyglactin sutures were coated with AgNPs using the dip-coating method. The uniform coating and morphology of AgNPs on the suture surface were confirmed using scanning electron microscopy (SEM). Each type of suture - polyglactin plain, antibiotic coated (Triclosan), AgNP coated, and dually coated (antibiotic and AgNP) - was assessed for their antibacterial properties against Gram-positive bacteria, Gram-negative bacteria, and anaerobes. These sutures were utilized in an abdominal and systemic inflammatory mice model for ileal anastomosis. The intestinal tissue was evaluated for acute and chronic inflammation and collagen deposition to assess the healing and inflammatory response.
The SEM and energy dispersive X-ray analysis showed successful coating of AgNPs on plain and antibiotic-coated sutures. In comparison with the other groups, the dually coated suture had the best antibacterial efficacy. The AgNP-coated sutures were able to decrease both acute and chronic inflammatory cell infiltration, but the collagen synthesis and deposition were enhanced.
AgNPs can be coated on Polyglactin suture either alone or in combination with antibiotics with preserved antibacterial effects. The dual coating of sutures gives a synergistic antibacterial effect. The AgNP diminishes immune response in the presence of preserved extracellular matrix generation.
由于银纳米颗粒（AgNPs）具有抗菌和抗炎特性，其在生物医学领域的应用已大量涌现。我们假设，将AgNPs与抗生素结合用于缝合线涂层，将增强缝合线的抗菌性能，同时还具有AgNPs对组织愈合的免疫调节作用这一额外优势。
采用浸涂法在聚乙醇酸缝合线上涂覆AgNPs。使用扫描电子显微镜（SEM）确认AgNPs在缝合线表面的均匀涂层和形态。评估每种类型的缝合线——普通聚乙醇酸缝合线、抗生素涂层（三氯生）缝合线、AgNP涂层缝合线和双重涂层（抗生素和AgNP）缝合线——对革兰氏阳性菌、革兰氏阴性菌和厌氧菌的抗菌性能。将这些缝合线用于腹部和全身炎症的小鼠回肠吻合模型。对肠道组织进行急性和慢性炎症以及胶原蛋白沉积评估，以评估愈合和炎症反应。
SEM和能量色散X射线分析表明，AgNPs成功涂覆在普通和抗生素涂层缝合线上。与其他组相比，双重涂层缝合线具有最佳的抗菌效果。AgNP涂层缝合线能够减少急性和慢性炎症细胞浸润，但增强了胶原蛋白的合成和沉积。
AgNPs可以单独或与抗生素联合涂覆在聚乙醇酸缝合线上，并保留抗菌效果。缝合线的双重涂层具有协同抗菌作用。在保留细胞外基质生成的情况下，AgNP可减少免疫反应。
