Chen Shu, Meng Gaoqiang, Wang Xing, Jiang Hemin, Shen Min, Wang Xueqin, Wang Chunhua
Department of Endocrinology, Nantong First People's Hospital, Affiliated Hospital 2 of Nantong University, No. 666 of Shengli Road, Chongchuan District, Nantong, 226014, Jiangsu , China.
Department of Neurosurgery, Nantong First People's Hospital, Affiliated Hospital 2 of Nantong University, Nantong, 226014, Jiangsu , China.
Diabetol Metab Syndr. 2024 Dec 19;16(1):305. doi: 10.1186/s13098-024-01549-9.
This study aims to examine the alterations and clinical significance of CD8 regulatory T cell subsets in the peripheral blood of individuals with type 1 diabetes mellitus (T1DM).
From January 2020 to December 2023, a study was conducted involving 40 individuals with T1DM, who visited the Department of Endocrinology at the First Affiliated Hospital of Nanjing Medical University (T1DM group). For comparison, 40 healthy individuals who underwent routine physical examinations at the same hospital during this period were selected as the control group. Peripheral blood mononuclear cells were isolated, and CD8 T cells were labeled with CD3, CD25 and FoxP3 to analyze their subset frequencies using flow cytometry. The study examined differences in subset frequencies between the two groups and explored correlations between subset frequency, disease duration, and age of onset.
The frequencies of CD8 CD25, CD8 FoxP3, CD8 CD25 CD3 and CD8 FoxP3 CD3 subsets in peripheral blood mononuclear cells did not significantly differ between the healthy control group and the T1DM group (P > 0.05). In the T1DM group, the expression level of CD25 on CD8 T cells showed no correlation with the age of onset or disease duration, and FoxP3 levels were also unrelated to the age of onset, with no statistical differences (P > 0.05). However, within the T1DM group, FoxP3 levels progressively decreased with longer disease duration, demonstrating a statistically significant negative correlation (Pearson r = -0.331, P < 0.05). In the T1DM group, the level of CD3 CD8 T cells expressed CD25, and there was no correlation between Foxp 3 level and age of onset, not statistically significant (P > 0.05), but the level of Foxp 3 in the T1DM group decreased with the duration of the disease, (Pearson r= - 0.363, P < 0.05).
The levels of CD8FoxP3 regulatory T cells in peripheral blood mononuclear cells of patients with T1DM show a significant correlation with disease duration, suggesting that these cells may play a critical role in the progression of T1DM.
本研究旨在探讨1型糖尿病（T1DM）患者外周血中CD8调节性T细胞亚群的变化及其临床意义。
2020年1月至2023年12月，对40例T1DM患者进行了研究，这些患者就诊于南京医科大学第一附属医院内分泌科（T1DM组）。作为对照，选取同期在该院进行常规体检的40例健康个体作为对照组。分离外周血单个核细胞，用CD3、CD25和FoxP3标记CD8 T细胞，采用流式细胞术分析其亚群频率。本研究检测了两组之间亚群频率的差异，并探讨了亚群频率、病程和发病年龄之间的相关性。
健康对照组和T1DM组外周血单个核细胞中CD8 CD25、CD8 FoxP3、CD8 CD25 CD3和CD8 FoxP3 CD3亚群的频率无显著差异（P>0.05）。在T1DM组中，CD8 T细胞上CD25的表达水平与发病年龄或病程无关，FoxP3水平也与发病年龄无关，无统计学差异（P>0.05）。然而，在T1DM组中，FoxP3水平随病程延长而逐渐降低，呈现出统计学显著的负相关（Pearson r = -0.331，P<0.05）。在T1DM组中，CD3 CD8 T细胞表达CD25的水平，Foxp 3水平与发病年龄之间无相关性，无统计学意义（P>0.05），但T1DM组中Foxp 3水平随疾病持续时间而降低，（Pearson r = -0.363，P<0.05）。
T1DM患者外周血单个核细胞中CD8FoxP3调节性T细胞水平与病程显著相关，提示这些细胞可能在T1DM的进展中起关键作用。
