Li Caijuan, Li Hao, Liang Yufan, Miao Rong, Liu Ziliang, Chen Sijin, Zhang Dan, Wang Cuiling, Wu Jiling, Zhou Xiangyan, Li Mengfei
State Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
State Key Laboratory of Aridland Crop Science/Agronomy College, Gansu Agricultural University, Lanzhou, 730070, China.
BMC Genomics. 2024 Dec 18;25(1):1204. doi: 10.1186/s12864-024-11129-w.
O-Methyltransferase (OMTs) is a class of conserved multifunctional enzymes that play important roles in plant developmental regulation, hormone signaling, secondary metabolite synthesis and abiotic stress response. The GiOMT gene family has been identified and analyzed in species such as citrus, alfalfa, Populus and grape, but has not been reported in Glycyrrhiza inflata Bat.
In this study, we systematically identified and analyzed the GiOMT gene family of G. inflata by bioinformatics, and analyzed their physicochemical properties, conserved motifs, conserved structural domains, gene structures, phylogenetic relationships, chromosomal localization and fragment duplications, and the expression patterns of GiOMT genes in combination with transcriptomic data and qRT-PCR. The results showed that a total of 41 GiOMTs were identified in G. inflata, which were named GiOMT1 ~ GiOMT41 based on their chromosomal locations. Protein characterization showed that 29 GiOMT proteins were hydrophilic and 12 GiOMT proteins were hydrophobic. Subcellular predicted localization revealed that most GiOMT proteins localized in the cytoplasm and chloroplasts. Phylogenetic relationships showed that the OMT genes of three species, G. inflata, Arabidopsis and alfalfa, were distributed in three taxa, while the GiOMT genes were distributed in taxa I and II. Promoters of GiOMT genes contained light responsive element and many hormone responsive elements. The expression levels of GiOMT genes under UV-B stress were varied, indicating that GiOMT gene was in response to abiotic stresses in G. inflata.
In this study, we investigated the genome-wide identification, structure, evolution and expression analysis of the GiOMT gene in G. inflata. The basal sequence of GiOMT genes was highly conserved throughout the evolutionary history of G. inflata. Most of the GiOMT genes were highly expressed in roots and were involved in the response to UV-B stress. The GiOMT genes may lead to the accumulation of flavonoids and enhancement of G. inflata quality and drug activity in G. inflata under UV-B radiation.
O-甲基转移酶（OMTs）是一类保守的多功能酶，在植物发育调控、激素信号传导、次生代谢物合成和非生物胁迫响应中发挥重要作用。已在柑橘、苜蓿、杨树和葡萄等物种中鉴定并分析了GiOMT基因家族，但在胀果甘草中尚未见报道。
本研究通过生物信息学方法对胀果甘草的GiOMT基因家族进行了系统鉴定和分析，并分析了它们的理化性质、保守基序、保守结构域、基因结构、系统发育关系、染色体定位和片段重复情况，以及结合转录组数据和qRT-PCR分析了GiOMT基因的表达模式。结果表明，在胀果甘草中共鉴定出41个GiOMT，根据其染色体位置命名为GiOMT1~GiOMT41。蛋白质特性分析表明，29个GiOMT蛋白为亲水性，12个GiOMT蛋白为疏水性。亚细胞预测定位显示，大多数GiOMT蛋白定位于细胞质和叶绿体中。系统发育关系表明，胀果甘草、拟南芥和苜蓿三个物种的OMT基因分布在三个分类群中，而GiOMT基因分布在分类群I和II中。GiOMT基因的启动子包含光响应元件和许多激素响应元件。UV-B胁迫下GiOMT基因的表达水平各不相同，表明GiOMT基因参与了胀果甘草对非生物胁迫的响应。
本研究对胀果甘草中GiOMT基因进行了全基因组鉴定、结构、进化和表达分析。GiOMT基因的基础序列在胀果甘草的整个进化历史中高度保守。大多数GiOMT基因在根中高表达，并参与对UV-B胁迫的响应。GiOMT基因可能导致胀果甘草在UV-B辐射下黄酮类化合物的积累以及胀果甘草品质和药用活性的提高。
