Zhang Mengyue, Yang Chunting, Gao Lingling, Zhao Yuanyuan, Shi Hongzhi
College of Tobacco Science, Henan Agricultural University, Zhengzhou, China.
Henan Academy of Agricultural Sciences, Zhengzhou, China.
Tob Induc Dis. 2024 Dec 18;22. doi: 10.18332/tid/196677. eCollection 2024.
This study aimed to evaluate the metabolomic profiles of urine samples obtained from smokers who smoked cigarettes with low and high nicotine content.
Three smokers participated in this study. They were given low-nicotine (LN) cigarettes, and urine was collected at the end of the third day for the LN group. After 1 week of not smoking, they were given high-nicotine (HN) cigarettes, and urine was collected for the HN group. Untargeted metabolomics and bioinformatic analysis methods were used for urine analysis.
PCA showed a high degree of similarity between samples within the group and a large distance between samples between groups, indicating a significant difference between the two groups. A total of 1150 significantly differential metabolites were selected between the HN and LN groups, such as cotinine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol-N-glucuronide. Two-way hierarchical clustering analysis also suggested noticeable differences between the two comparison groups Enrichment analysis indicates that the differential metabolites between the two groups were mainly enriched in 19 pathways, such as the protein kinase G (cGMP)-protein kinase G (PKG) signaling pathway, adenosine monophosphate (AMP)-activated protein kinase signaling pathway, mammalian target of rapamycin signaling pathway, and Parkinson's disease.
Cigarettes with different nicotine content may alter the metabolism of smokers. A total of 1150 significantly different metabolites were identified between the HN and LN groups, which were mainly enriched in ABC transporters, protein kinase G (cGMP)-protein kinase G (PKG) signaling pathway, caffeine metabolism, and arginine biosynthesis pathways.
本研究旨在评估从吸食低尼古丁含量和高尼古丁含量香烟的吸烟者中获取的尿液样本的代谢组学特征。
三名吸烟者参与了本研究。他们被给予低尼古丁（LN）香烟，LN组在第三天结束时收集尿液。在一周不吸烟后，他们被给予高尼古丁（HN）香烟，HN组收集尿液。采用非靶向代谢组学和生物信息学分析方法对尿液进行分析。
主成分分析（PCA）显示组内样本之间具有高度相似性，而组间样本之间距离较大，表明两组之间存在显著差异。在HN组和LN组之间共筛选出1150种显著差异代谢物，如可替宁和4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁醇-N-葡萄糖醛酸。双向层次聚类分析也表明两个比较组之间存在明显差异。富集分析表明，两组之间的差异代谢物主要富集在19条通路中，如蛋白激酶G（cGMP）-蛋白激酶G（PKG）信号通路、单磷酸腺苷（AMP）激活的蛋白激酶信号通路、雷帕霉素靶蛋白信号通路和帕金森病。
不同尼古丁含量的香烟可能会改变吸烟者的代谢。在HN组和LN组之间共鉴定出1150种显著不同的代谢物，它们主要富集在ABC转运蛋白、蛋白激酶G（cGMP）-蛋白激酶G（PKG）信号通路、咖啡因代谢和精氨酸生物合成途径中。
