Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, New York 14260, United States.
Department of Thoracic Surgery, Roswell Park Comprehensive Cancer Center, Elm and Carlton Street, Buffalo, New York 14263, United States.
ACS Nano. 2023 May 9;17(9):8108-8122. doi: 10.1021/acsnano.2c10970. Epub 2023 Apr 27.
Tumor derived exosomes (TEXs) have emerged as promising biomarkers for cancer liquid biopsy. Conventional methods (such as ELISA and qRT-PCR) and emerging biosensing technologies mainly detect a single type of exosomal biomarker due to the distinct properties of different biomolecules. Sensitive detection of two different types of TEX biomarkers, i.e., protein and microRNA combined biomarkers, may greatly improve cancer diagnostic accuracy. We developed an exosome protein microRNA one-stop (Exo-PROS) biosensor that not only selectively captured TEXs but also enabled , simultaneous detection of TEX protein-microRNA pairs via a surface plasmon resonance mechanism. Exo-PROS assay is a fast, reliable, low sample consumption, and user-friendly test. With a total of 175 cancer patients and normal controls, we demonstrated that TEX protein-microRNA pairs measured by Exo-PROS assay detected lung cancer and breast cancer with 99% and 96% accuracy, respectively. Exo-PROS assay also showed superior diagnostic performance to conventional ELISA and qRT-PCR methods. Our results demonstrated that Exo-PROS assay is a potent liquid biopsy assay for cancer diagnosis.
肿瘤来源的外泌体（TEXs）已经成为癌症液体活检有前途的生物标志物。由于不同生物分子的独特性质，传统方法（如 ELISA 和 qRT-PCR）和新兴的生物传感技术主要检测单一类型的外泌体生物标志物。敏感检测两种不同类型的 TEX 生物标志物，即蛋白质和 microRNA 联合生物标志物，可能极大地提高癌症诊断的准确性。我们开发了一种外泌体蛋白 microRNA 一站式（Exo-PROS）生物传感器，不仅可以选择性地捕获 TEXs，还可以通过表面等离子体共振机制同时检测 TEX 蛋白-microRNA 对。Exo-PROS 检测法是一种快速、可靠、低样本消耗和用户友好的测试。通过对 175 名癌症患者和正常对照组的检测，我们证明了 Exo-PROS 检测法检测的 TEX 蛋白-microRNA 对检测肺癌和乳腺癌的准确率分别为 99%和 96%。Exo-PROS 检测法也显示出优于传统 ELISA 和 qRT-PCR 方法的诊断性能。我们的研究结果表明，Exo-PROS 检测法是一种用于癌症诊断的有力的液体活检检测法。
