Cho Eui-Sang, Hwang Chi Young, Seo Myung-Ji
BioTechnology Institute, University of Minnesota, St. Paul, MN, 55108, USA.
Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
Bioresour Bioprocess. 2024 Dec 19;11(1):111. doi: 10.1186/s40643-024-00834-9.
Haloarchaea represents a unique group of microorganisms that have adapted to thrive in high-salt environments. These microbes produce distinctive biomolecules, some of which exhibit extraordinary properties. One such biomolecule is bacterioruberin, a prominent red-pigmented C carotenoid commonly found in halophilic archaea, renowned for its antioxidant properties and potential as a functional resource. This study aimed to enhance the culture conditions for optimal production of C carotenoids, primarily bacterioruberin, using "Haloferax marinum" MBLA0078. The optimization process involved a combination of one-factor-at-a-time (OFAT) and statistical methodology. Under OFAT-optimized conditions, fed-batch fermentation, and response surface methodology (RSM) optimization, carotenoid production reached 0.954 mg/L, 2.80 mg/L, and 2.16 mg/L, respectively, in a 7-L laboratory-scale fermenter. Notably, RSM-optimized conditions led to a 12-fold increase in productivity (0.72 mg/L/day) compared to the basal DBCM2 medium (0.06 mg/L/day). These findings suggest that strain MBLA0078 holds significant promise for commercial-scale production of bacterioruberin.
嗜盐古菌是一类独特的微生物群体，它们已适应在高盐环境中茁壮成长。这些微生物会产生独特的生物分子，其中一些具有非凡的特性。一种这样的生物分子是细菌红素，它是一种在嗜盐古菌中常见的显著的红色C类胡萝卜素，以其抗氧化特性和作为功能资源的潜力而闻名。本研究旨在利用“海生嗜盐嗜盐菌”MBLA0078优化培养条件，以实现C类胡萝卜素（主要是细菌红素）的最佳产量。优化过程涉及单因素法（OFAT）和统计方法的结合。在OFAT优化条件、补料分批发酵和响应面法（RSM）优化下，在7升实验室规模发酵罐中，类胡萝卜素产量分别达到0.954毫克/升、2.80毫克/升和2.16毫克/升。值得注意的是，与基础DBCM2培养基（0.06毫克/升/天）相比，RSM优化条件使生产力提高了12倍（0.72毫克/升/天）。这些发现表明，菌株MBLA0078在细菌红素的商业规模生产方面具有巨大潜力。
