Hepner Sabrina, Jolley Keith A, Castillo-Ramirez Santiago, Mourkas Evangelos, Dangel Alexandra, Wieser Andreas, Hübner Johannes, Sing Andreas, Fingerle Volker, Margos Gabriele
German National Reference Centre for Borrelia, Oberschleissheim, Germany; Bavarian Health and Food Safety Authority, Oberschleissheim, Germany.
Department of Biology, University of Oxford, Oxford, UK.
Cell Rep Methods. 2025 Jan 27;5(1):100935. doi: 10.1016/j.crmeth.2024.100935. Epub 2024 Dec 18.
Multi-locus sequence typing (MLST) based on eight genes has become the method of choice for Borrelia typing and is extensively used for population studies. Whole-genome sequencing enables studies to scale up to genomic levels but necessitates extended schemes. We have developed a 639-loci core genome MLST (cgMLST) scheme for Borrelia burgdorferi sensu lato (s.l.) that enables unambiguous genotyping and improves the robustness of phylogenies and lineage resolution within species. Notably, all inner nodes of the cgMLST phylogenies had consistently high statistical support. Analyses of the genetically homogeneous European B. bavariensis population support the notion that cgMLST provides high discriminatory power even for closely related isolates. While isolates differed maximally in one MLST locus, there were up to 179 cgMLST loci differences. Thus, the developed cgMLST scheme for B. burgdorferi s.l. resolves lineages at a finer resolution than MLST and improves insights into the evolutionary history of the species complex.
基于八个基因的多位点序列分型（MLST）已成为疏螺旋体分型的首选方法，并广泛用于群体研究。全基因组测序使研究能够扩展到基因组水平，但需要更复杂的方案。我们为广义伯氏疏螺旋体（Borrelia burgdorferi sensu lato，s.l.）开发了一种639个位点的核心基因组MLST（cgMLST）方案，该方案能够进行明确的基因分型，并提高了系统发育和物种内谱系分辨率的稳健性。值得注意的是，cgMLST系统发育树的所有内部节点都始终具有较高的统计支持率。对基因同质的欧洲巴伐利亚疏螺旋体群体的分析支持了这样一种观点，即cgMLST即使对于密切相关的分离株也具有很高的鉴别力。虽然分离株在一个MLST位点上的差异最大，但cgMLST位点差异多达179个。因此，为广义伯氏疏螺旋体开发的cgMLST方案比MLST能以更高的分辨率解析谱系，并增进对该物种复合体进化历史的了解。
