Luo Ying, Jia Xiaoli, Wu Xiaozhuo, Diao Ling, Zhao Yawei, Liu Xing, Peng Yi, Zhong Wen, Xing Malcolm, Lyu Guozhong
Burn & Trauma Treatment Center, The Affiliated Hospital of Jiangnan University, Wuxi, 214000, China.
Engineering Research Center of the Ministry of Education for Wound Repair Technology, Jiangnan University, Wuxi, 214000, China.
J Nanobiotechnology. 2024 Dec 19;22(1):781. doi: 10.1186/s12951-024-03056-5.
Bacterial infections and antibiotic resistance represent significant global public health challenges, necessitating the development of innovative antibacterial agents with targeted delivery capabilities. Our study utilized macrophages' natural ability to recognize bacteria and the increased reactive oxygen species (ROS) at infection sites to develop a novel nanoparticle for targeted delivery and controlled release. We prepared bacteria-activated macrophage membranes triggered by Staphylococcus aureus (Sa-MMs), which showed significantly higher expression of Toll-like receptors (TLRs), compared to normal macrophage membranes (MMs). These Sa-MMs were then used to coat vancomycin-loaded amphiphilic nanoparticles with ROS responsiveness (Van-NPs), resulting in the novel targeted delivery system Sa-MM@Van-NPs. Studies both In vitro and in vivo demonstrated that biocompatible Sa-MM@Van-NPs efficiently targeted infected sites and released vancomycin to eliminate bacteria, facilitating faster wound healing. By combining targeted delivery to infected sites and ROS-responsive antibiotic release, this approach might represent a robust strategy for precise infection eradication and enhanced wound healing.
细菌感染和抗生素耐药性是重大的全球公共卫生挑战，因此需要开发具有靶向递送能力的创新抗菌剂。我们的研究利用巨噬细胞识别细菌的天然能力以及感染部位活性氧（ROS）的增加，开发了一种用于靶向递送和控释的新型纳米颗粒。我们制备了由金黄色葡萄球菌触发的细菌激活巨噬细胞膜（Sa-MMs），与正常巨噬细胞膜（MMs）相比，其Toll样受体（TLRs）表达显著更高。然后将这些Sa-MMs用于包裹具有ROS响应性的载万古霉素两亲纳米颗粒（Van-NPs），从而形成新型靶向递送系统Sa-MM@Van-NPs。体外和体内研究均表明，生物相容性良好的Sa-MM@Van-NPs能有效靶向感染部位并释放万古霉素以消除细菌，促进伤口更快愈合。通过将感染部位的靶向递送与ROS响应性抗生素释放相结合，这种方法可能是一种精确根除感染和促进伤口愈合的有力策略。
