Lizondo-Aranda Paloma, Rodríguez-Muñiz Gemma M, Miranda Miguel A, Heyne Belinda, Lhiaubet-Vallet Virginie
Instituto Universitario Mixto de Tecnología Química (UPV-CSIC), Universitat Politècnica de València, Consejo Superior de Investigaciones Científicas, Avda de los Naranjos s/n, 46022, Valencia, Spain.
Department of Chemistry, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Photochem Photobiol Sci. 2025 Jan;24(1):1-12. doi: 10.1007/s43630-024-00663-x. Epub 2024 Dec 19.
Photophysical and photochemical studies were carried out to examine the photoreactivity of etheno adducts, 1,N-ethenoadenine (εdA) and 1,N-ethenoguanine (εdG), in the presence of two well-known photosensitizers acting by Type I and/or Type II mechanisms such as 4-carboxybenzophenone (CBP) and rose Bengal (RB), respectively. Steady-state photolysis experiments combined with HPLC and mass spectroscopy measurements lead to photoproducts that correspond to the repaired nucleosides. To determine the mechanism of this photooxidation processes, phosphorescence spectroscopy, direct detection of singlet oxygen luminescence and laser flash photolysis were carried out. This work establishes that εdG and εdA are sensitive to both types of processes (Type I and II).
进行了光物理和光化学研究，以考察乙烯基加合物1,N-乙烯基腺嘌呤（εdA）和1,N-乙烯基鸟嘌呤（εdG）在两种分别通过I型和/或II型机制起作用的著名光敏剂存在下的光反应性，这两种光敏剂分别是4-羧基二苯甲酮（CBP）和孟加拉玫瑰红（RB）。结合高效液相色谱和质谱测量的稳态光解实验产生了与修复的核苷相对应的光产物。为了确定这种光氧化过程的机制，进行了磷光光谱、单线态氧发光的直接检测和激光闪光光解。这项工作证实εdG和εdA对这两种类型的过程（I型和II型）均敏感。
