Slivka Joseph, Yildiz Ahmet
Department of Physics, University of California, Berkeley, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Methods Mol Biol. 2025;2881:133-144. doi: 10.1007/978-1-0716-4280-1_6.
Molecular motors move processively along cytoskeletal filaments by stepping of their motor domains (MDs). Observation of how the MDs step relative to each other reveals the mechanism of motor processivity and various gating mechanisms used by motors to coordinate the catalytic cycles of their MDs. This chapter will discuss developments in simultaneous observation of the stepping motions of the two MDs of processive motors using two-color single-particle tracking microscopy.Techniques presented: FIONA, multicolor tracking/image registration.
分子马达通过其马达结构域（MDs）的步移沿着细胞骨架丝进行连续移动。观察MDs彼此之间的步移方式可揭示马达连续移动的机制以及马达用于协调其MDs催化循环的各种门控机制。本章将讨论使用双色单粒子跟踪显微镜同时观察连续马达的两个MDs步移运动方面的进展。介绍的技术：荧光成像纳米显微镜、多色跟踪/图像配准。
