Wang Zekai, Qu Lingli, Yang Yu'e, Cui Weikang, Gu Yangyang, Wang Haibo, Pan Hongzhi
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Shanghai University of Medicine & Health Sciences, Shanghai, 201318, China.
Mikrochim Acta. 2024 Dec 22;192(1):26. doi: 10.1007/s00604-024-06870-8.
A novel electrochemiluminescence (ECL) biosensor was developed for the ultrasensitive detection of miRNA-155, based on the synergistic combination of multifunctional nanomaterials. The biosensor employed a conductive metal-organic framework (MOF), Ni(HAB) (HAB = hexaaminobenzene), as the substrate material. The unique π-electron conjugated structure of Ni(HAB) endowed the biosensor with excellent electron transport properties, significantly enhancing its sensitivity. Furthermore, the innovative preparation of Au@ZnNiAl-LDH nanocomposites, characterized by a high specific surface area was employed to synergistically enhance the catalytic performance of the biosensor in conjunction with Ni(HAB). The Au@ZnNiAl-LDH also provided stable anchoring sites for the capture unit, comprised of a DNA tetrahedron hairpin composite structure (DT-HP). Additionally, a porous aluminum-based metal-organic framework (MIL-53(Al)-NH) was utilized to encapsulate Ru(bpy), constructing a Ru@MIL-53(Al)-NH signal unit that effectively improved the stability of the ECL signal. Under optimal conditions, the ECL intensity of the biosensor exhibited a robust linear relationship with the logarithm of miRNA-155 concentration over a range 3 fM to 1 nM, achieving a detection limit as low as 0.9 fM. Moreover, the biosensor demonstrated exceptional specificity, selectivity, and stability, highlighting its significant potential for applications in bioanalysis and clinical diagnosis, particularly for the early diagnosis of tumor.
基于多功能纳米材料的协同组合，开发了一种用于超灵敏检测miRNA - 155的新型电化学发光（ECL）生物传感器。该生物传感器采用导电金属有机框架（MOF）Ni(HAB)（HAB = 六氨基苯）作为基底材料。Ni(HAB)独特的π电子共轭结构赋予生物传感器优异的电子传输性能，显著提高了其灵敏度。此外，还采用了具有高比表面积的Au@ZnNiAl-LDH纳米复合材料的创新制备方法，与Ni(HAB)协同增强生物传感器的催化性能。Au@ZnNiAl-LDH还为捕获单元（由DNA四面体发夹复合结构（DT-HP）组成）提供了稳定的锚定位点。此外，利用多孔铝基金属有机框架（MIL-53(Al)-NH）封装Ru(bpy)，构建了Ru@MIL-53(Al)-NH信号单元，有效提高了ECL信号的稳定性。在最佳条件下，生物传感器的ECL强度与miRNA - 155浓度的对数在3 fM至1 nM范围内呈现出稳健的线性关系，检测限低至0.9 fM。此外，该生物传感器表现出优异的特异性、选择性和稳定性，突出了其在生物分析和临床诊断，特别是肿瘤早期诊断中的巨大应用潜力。
