Wound healing potential of mouth gel containing isopimarane diterpene from rhizomes for treatment of oral stomatitis.

BACKGROUND

Oral ulcers have an impact on 25% of the global population including patients who are suffering from chemotherapy and radiotherapy treatments. L. has been traditionally used for treatment of mouth sores and tongue blisters. However, the wound healing study of isopimarane diterpenes isolated from is still limited.

OBJECTIVE

This study aims to evaluate the wound healing potential of 6β-acetoxysandaracopimaradiene-1α,9α-diol (KG), a compound isolated from , by examining its biological activities. Additionally, we investigate the physicochemical and biological properties of (KG) in formulated mouth gels.

METHODS

The KG mouth gels at 0.10%, 0.25% and 0.50% w/w were formulated using sodium carboxymethylcellulose as a gelling agent, and their physicochemical and biological stabilities were assessed through a heating-cooling acceleration test. The quantification of KG contents in KG mouth gels was determined using gas chromatography. Both KG and KG mouth gels were evaluated for their wound healing properties including cell proliferation, cell migration, and antioxidant activity (HO-induced oxidative stress) in human gingival fibroblast (HGF-1-ATCC CRL-2014) (HGF-1). In addition, the anti-inflammatory activity against nitric oxide (NO) production was investigated in macrophage cells (RAW 264.7).

RESULTS

After KG mouth gels were incubated under heating-cooling acceleration condition, the physicochemical properties of the KG mouth gels remain stable across various parameters, including appearance, color, smell, texture, pH, viscosity, separation, and KG content. The biological studies indicated that the KG compound possessed good wound healing potential. The 0.50% KG mouth gel exhibited marked anti-inflammatory effect by inhibiting NO production with an IC of 557.7 µg/ml, comparable to that of Khaolaor mouth gel, a positive control. The 0.25% KG mouth gel increased HGF-1 cell proliferation to 101.7-103.5%, whereas all formulations of KG mouth gel enhanced HGF-1 cell migration to 94.7-98.9%, higher than Khaolaor mouth gel (73.5%). Moreover, 0.50% KG mouth gel also showed a good antioxidant effect under HO-induced oxidative stress.

CONCLUSION

This study substantiates the significant biological activities related to the wound healing property of 0.50% KG mouth gel for treatment of aphthous ulcers and oral stomatitis from chemotherapy and radiotherapy treatments.