Narayana M S Sandeep Veda, Rajesh N, Dastagiri C, Mobeen S Anjum, Khadri Habeeb, Chandrasekhar T, Prasanna V Anu, Riazunnisa K
Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa, Andhra Pradesh, India.
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim, Kingdom of Saudi Arabia.
Chem Biodivers. 2025 May;22(5):e202402361. doi: 10.1002/cbdv.202402361. Epub 2025 Jan 7.
The rapid growth of nanotechnology has opened new frontiers in biomedical applications, particularly through the use of metal nanoparticles. This study explores the green synthesis of copper nanoparticles (CuNPs) using an aqueous extract of Pleurotus ostreatus (PO-CuNPs) and their characterization through UV-visible spectroscopy, FTIR, SEM, and EDAX. The synthesized PO-CuNPs demonstrated exceptional antioxidant activity, evident in hydrogen peroxide scavenging and phosphomolybdenum assays. Their antibacterial efficacy was significant against Bacillus subtilis (18 ± 0.11 mm inhibition zone) and moderate against other bacterial strains. The antidiabetic potential of PO-CuNPs was confirmed by α-amylase (82%) and β-glucosidase (86%) inhibition assays. Molecular docking studies revealed kaempferol (-9.0 kcal) and quercetin (-9.2 kcal) as potent α-amylase inhibitors, while myricetin (-8.4 kcal) was most effective against β-glucosidase due to its favorable interactions. Despite high scores, rutin was excluded due to poor drug-likeliness, highlighting kaempferol and myricetin as promising antidiabetic agents. This research highlights the promising biomedical applications of P. ostreatus-based CuNPs, particularly in managing oxidative stress, microbial infections, and diabetes, showcasing their potential as eco-friendly therapeutic agents.
纳米技术的迅速发展为生物医学应用开辟了新的领域，特别是通过金属纳米颗粒的使用。本研究探索了利用平菇水提取物绿色合成铜纳米颗粒（PO-CuNPs），并通过紫外可见光谱、傅里叶变换红外光谱、扫描电子显微镜和能谱分析对其进行表征。合成的PO-CuNPs表现出优异的抗氧化活性，在过氧化氢清除和磷钼酸测定中表现明显。它们对枯草芽孢杆菌具有显著的抗菌效果（抑菌圈为18±0.11毫米），对其他细菌菌株的抗菌效果中等。通过α-淀粉酶（82%）和β-葡萄糖苷酶（86%）抑制试验证实了PO-CuNPs的抗糖尿病潜力。分子对接研究表明，山奈酚（-9.0千卡）和槲皮素（-9.2千卡）是有效的α-淀粉酶抑制剂，而杨梅素（-8.4千卡）由于其良好的相互作用对β-葡萄糖苷酶最有效。尽管得分较高，但芦丁因药物相似性较差而被排除，突出了山奈酚和杨梅素作为有前景的抗糖尿病药物。本研究突出了基于平菇的铜纳米颗粒在生物医学方面的应用前景，特别是在管理氧化应激、微生物感染和糖尿病方面，展示了它们作为生态友好型治疗剂的潜力。
