一种基于真菌的铜纳米粒子合成方法，用于测定其抗癌、抗糖尿病和抗菌活性。

A fungal based synthesis method for copper nanoparticles with the determination of anticancer, antidiabetic and antibacterial activities.

机构信息

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University Multan, Pakistan.

Department of Biotechnology, CECOS University of Information Technology and Emerging Sciences, Peshawar, Pakistan.

出版信息

J Microbiol Methods. 2020 Jul;174:105966. doi: 10.1016/j.mimet.2020.105966. Epub 2020 May 29.

DOI:10.1016/j.mimet.2020.105966

PMID:32474053

Abstract

BACKGROUND

Biological procedures for the synthesis of copper nanoparticles (CuNPs) are eco-friendly, cost-effective, and easily scalable as compared to chemical and physical methods.

METHODS

In the present study, a simple fungus based synthesis method was used for copper nanoparticles. After morphological and molecular characterization of fungal strains, Aspergillus niger strain STA9 was used for CuNPs synthesis. Particles synthesized by fungi were characterized by UV-visible spectrophotometer, FTIR, and Zetasizer. The MTT anti-cancer, anti-diabetic and antibacterial assays of CuNPs were performed.

RESULTS

The CuNPs were produced at optimized conditions with a size of 500 nm, Z-average 398.2 nm, and 0.246 poly dispersion index. These particles were quantified at 480 nm and FTIR confirmed the existence of OH and -C=C- functional groups. MTT assay revealed that CuNPs have a significant cytotoxic effect against human hepatocellular carcinoma cell lines (Huh-7) with 3.09 μg/ml IC50 value. Alpha-glucosidase inhibition showed that CuNPs have a moderate antidiabetic effect. The agar well antibacterial effect indicated 19, 21, 16, 20, and 17 mm zone of inhibition against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Micrococcus luteus and Bacillus subtilis respectively.

CONCLUSION

Such biomedical applications of CuNPs reveal the importance of a targeted drug delivery system.

摘要

背景

与化学和物理方法相比，生物法合成铜纳米粒子（CuNPs）具有环保、经济高效且易于规模化等优点。

方法

本研究采用一种简单的真菌合成法来制备铜纳米粒子。在对真菌菌株进行形态学和分子鉴定后，选用黑曲霉（Aspergillus niger）STA9 菌株来合成 CuNPs。采用紫外-可见分光光度计、傅里叶变换红外光谱（FTIR）和 Zetasizer 对真菌合成的颗粒进行了表征。还进行了 CuNPs 的 MTT 抗癌、抗糖尿病和抗菌测定。

结果

在优化条件下生成的 CuNPs 粒径为 500nm，Z-平均粒径为 398.2nm，多分散指数为 0.246。这些颗粒在 480nm 处被定量，FTIR 证实了 OH 和 -C=C- 官能团的存在。MTT 测定表明，CuNPs 对人肝癌细胞系（Huh-7）具有显著的细胞毒性，IC50 值为 3.09μg/ml。α-葡萄糖苷酶抑制试验表明，CuNPs 具有中度的抗糖尿病作用。琼脂孔抗菌试验表明，CuNPs 对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、藤黄微球菌和枯草芽孢杆菌的抑菌圈直径分别为 19、21、16、20 和 17mm。