Devale Ajit, Nikalaje Samadhan, Prasad Neeraj R, Varale Amit
Department of Chemistry, A.S.P. College, Devrukh Dist, Ratnagiri, Maharashtra State, India.
Department of Chemistry, A.C.S. College, Lanja Dist, Ratnagiri, Maharashtra State, India.
Discov Nano. 2025 Sep 7;20(1):153. doi: 10.1186/s11671-025-04300-z.
Herein, ruthenium nanoparticles (RuNPs) were synthesized using Tridax procumbens leaf extract as a reducing and stabilizing agent. The synthesis was optimized by adjusting temperature, leaf extract concentration, and reaction time. The synthesized RuNPs were characterized using UV-visible, XRD, EDAX, FTIR spectroscopy, SEM, and TEM, revealing uniform size and morphology. UV-visible spectroscopy confirmed RuNP formation with an absorption peak at 288 nm. FTIR analysis identified functional groups, with a peak at 600-800 cm indicating metallic Ru. XRD patterns showed peaks corresponding to RuNPs, with an average crystal size of 12.9 nm. SEM and TEM images revealed spherical RuNPs with an average diameter of 11.30 nm. The biological properties of the RuNPs were evaluated, demonstrating significant antibacterial and antifungal properties, and notable antioxidant activity. Antimicrobial activity was observed against Gram-positive bacteria (B. cereus, S. aureus) and Gram-negative bacteria (P. aeruginosa, E. coli) at concentrations of 50 µg/mL and above. The RuNPs showed antifungal activity against Candida albicans at 75 µg/ml and 100 µg/ml, but no activity against Aspergillus niger. The highest antioxidant activity was 77.13 ± 0.64% at a concentration of 100 µl. This study highlights the feasibility of utilizing Tridax procumbens leaf extract for the environmentally friendly synthesis of ruthenium nanoparticles, demonstrating their potential in biomedical applications and green chemistry.
在此，以三叶鬼针草叶提取物作为还原剂和稳定剂合成了钌纳米颗粒（RuNPs）。通过调节温度、叶提取物浓度和反应时间对合成过程进行了优化。使用紫外可见光谱、X射线衍射（XRD）、能量散射X射线光谱（EDAX）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对合成的RuNPs进行了表征，结果显示其尺寸和形态均一。紫外可见光谱证实了RuNP的形成，在288 nm处有一个吸收峰。FTIR分析确定了官能团，在600 - 800 cm处有一个峰表明存在金属Ru。XRD图谱显示出与RuNPs对应的峰，平均晶体尺寸为12.9 nm。SEM和TEM图像显示球形RuNPs，平均直径为11.30 nm。对RuNPs的生物学特性进行了评估，结果表明其具有显著的抗菌和抗真菌特性以及明显的抗氧化活性。在浓度为50 μg/mL及以上时，观察到对革兰氏阳性菌（蜡样芽孢杆菌、金黄色葡萄球菌）和革兰氏阴性菌（铜绿假单胞菌、大肠杆菌）有抗菌活性。RuNPs在75 μg/ml和100 μg/ml时对白色念珠菌显示出抗真菌活性，但对黑曲霉没有活性。在浓度为100 μl时，最高抗氧化活性为77.13±0.64%。本研究突出了利用三叶鬼针草叶提取物进行钌纳米颗粒环境友好合成的可行性，证明了它们在生物医学应用和绿色化学中的潜力。
