基于蛋白质组学对激光捕获显微切割的福尔马林固定石蜡包埋组织样本进行分析。
Proteomics-Based Analysis of Laser-Capture Micro-dissected, Formalin-Fixed Paraffin-Embedded Tissue Samples.
作者信息
Maia Teresa Mendes, Van Haver Delphi, Dufour Sara, Van der Linden Malaïka, Dendooven Amélie, Impens Francis, Devos Simon
机构信息
VIB-UGent Center for Medical Biotechnology, Ghent, Belgium.
Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.
出版信息
Methods Mol Biol. 2025;2884:333-354. doi: 10.1007/978-1-0716-4298-6_20.
The ability to bring spatial resolution to omics studies enables a deeper understanding of cell populations and interactions in biological tissues. In the case of proteomics, single-cell and spatial approaches have been particularly challenging, due to limitations in sensitivity and throughput relative to other omics fields. Recent developments at the level of sample handling, chromatography, and mass spectrometry have set the stage for proteomics to be established in these new disciplines.
将空间分辨率引入组学研究的能力，有助于更深入地了解生物组织中的细胞群体及其相互作用。就蛋白质组学而言，由于相对于其他组学领域在灵敏度和通量方面存在局限性，单细胞和空间分析方法一直颇具挑战。样品处理、色谱分析和质谱分析层面的最新进展，为蛋白质组学在这些新学科中的建立奠定了基础。
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