Trade-off movement between hydraulic resistance escape and shear stress escape by cancer cells.

In the circulatory system, the microenvironment surrounding cancer cells is complex and involves multiple coupled factors. We selected two core physical factors, shear stress and hydraulic resistance, and constructed a microfluidic device with dual negative inputs to study the trade-off movement behavior of cancer cells when facing coupled factors. We detected significant shear stress escape phenomena in the MDA-MB-231 cell line and qualitatively explained this behavior using a cellular force model. Through the dual validation of substrate anti-cell-adhesion modification and employment of the MCF-7 cell line, we further substantiated the predictability and feasibility of our model. This study provides an explanation for the trade-off underlying the direction-choosing mechanism of cancer cells when facing environmental selection.