对不同压力下癌细胞穿越血管行为的生物物理研究揭示了细胞运动状态的转变。
Biophysical studies of cancer cells' traverse-vessel behaviors under different pressures revealed cells' motion state transition.
机构信息
The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China.
Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
出版信息
Sci Rep. 2022 May 5;12(1):7392. doi: 10.1038/s41598-022-11047-5.
Circulating tumor cells (CTCs) survive in the bloodstream and then seed and invade to foster tumor metastasis. The arrest of cancer cells is favored by permissive flow forces and geometrical constraints. Through the use of high-throughput microfluidic devices designed to mimic capillary-sized vessels, we applied pressure differences to cancer cells (MCF-7 cell line) and recorded the cell traverse-vessel behaviors. Our results showed that cancer cells transform from a Newtonian droplet state to an adhesion/migration state when cancer cells traverse artificial vessels. To explain these phenomena, a modified Newtonian droplet model was also proposed. These phenomena and the modified model may reveal how CTCs in the blood seed and invade vessels under suitable conditions.
循环肿瘤细胞 (CTCs) 能够在血液中存活，然后定植和侵袭以促进肿瘤转移。癌细胞的停滞受到允许的流动力和几何约束的影响。通过使用设计用于模拟毛细血管大小的血管的高通量微流控装置，我们向癌细胞（MCF-7 细胞系）施加压力差，并记录细胞穿越血管的行为。我们的结果表明，当癌细胞穿越人工血管时，癌细胞从牛顿液滴状态转变为粘附/迁移状态。为了解释这些现象，还提出了一个改进的牛顿液滴模型。这些现象和改进的模型可能揭示了血液中的 CTC 如何在适当的条件下定植和侵袭血管。
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