Koltovskaia Sofia, Ohtao Akane, Kitaoka Motomitsu, Nakai Hiroyuki, Nihira Takanori
1 Graduate School of Science and Technology, Niigata University.
2 Faculty of Agriculture, Niigata University.
J Appl Glycosci (1999). 2024 Nov 20;71(4):123-125. doi: 10.5458/jag.jag.JAG-2024_0008. eCollection 2024.
β-Glucose 1-phosphate (βGlc1P) is a donor substrate in the synthesis of various α-glucosides by glycoside phosphorylases belonging to the glycoside hydrolase family 65. This study presents an efficient synthesis of βGlc1P combining enzymatic phosphorolysis of inexpensive maltose and baker's yeast fermentation to bias the equilibrium toward maltose phosphorolysis by removing released glucose. Mass production of βGlc1P was obtained in a 2 L reaction mixture initially containing 500 mM maltose and inorganic phosphate, with a yield of 76 %. βGlc1P was isolated from the reaction mixture by crystallization after electrodialysis to obtain 181 g of βGlc1P as a bis(cyclohexylammonium) salt.
β-葡萄糖-1-磷酸（βGlc1P）是糖苷水解酶家族65中的糖苷磷酸化酶合成各种α-糖苷的供体底物。本研究提出了一种高效合成βGlc1P的方法，该方法结合了廉价麦芽糖的酶促磷酸解和面包酵母发酵，通过去除释放的葡萄糖使平衡偏向麦芽糖磷酸解。在最初含有500 mM麦芽糖和无机磷酸盐的2 L反应混合物中实现了βGlc1P的大规模生产，产率为76%。通过电渗析后结晶从反应混合物中分离出βGlc1P，得到181 g作为双（环己基铵）盐的βGlc1P。
