Xiao Liuyue, Gong Huxuan, Tan Xiao, Chen Ping, Yang Yanjing, Zhu Hua, Zhong Shian
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
GuangXi University of Chinese Medicine, Nanning 530200, China.
Int J Biol Macromol. 2025 Feb;290:139095. doi: 10.1016/j.ijbiomac.2024.139095. Epub 2024 Dec 23.
CVP-2 is a homogeneous polysaccharide extracted from the whole plant of Christia vespertilionis, with an average molecular weight of approximately 92,920 Da. Its main chain consists of repeating units of [3,5)-α-L-Araf-(1] → [5)-α-L-Araf-(1]→, with branches at the C-3 position: branch 1 is α-L-Araf-(1→, and branch 2 is α-L-Araf-(1 → 4)-. Additionally, the structure includes β-D-Gclp-(1 → [4)-β-D-Glap-(1] → 5)-α-L-Araf-(1→. In vitro experiments conducted on cancer and normal cell lines demonstrated that the purified polysaccharide exhibited a significant pro-apoptotic effect on lung and liver cancer cells, while having no impact on the growth of normal tissue cells at dosage concentrations ranging from 125 to 1000 μg/mL. The inhibition of cell viability at 1000 μg/mL CVP-2 was 49.63 % for A549, 2.6 % for 16HBE, 28.56 % for HepG2, and 12.64 % for LX-2. Compared to the drug-free group, the death rates of A549, 16HBE, HepG2, and LX-2 cells increased by 37.13 %, 4.15 %, 26.00 %, and 4.41 %, respectively. CVP-2 was found to inhibit the G1/S phase of the cell cycle. The potential anticancer mechanism of CVP-2 involves a reduction in the Bcl-2/Bax protein in cancer cells, with no significant effect on the growth of normal cells. Therefore, CVP-2 holds great promise as a broad-spectrum antitumor agent and dietary supplement.
CVP - 2是从蝙蝠草全株中提取的一种均匀多糖，平均分子量约为92,920道尔顿。其主链由[3,5)-α - L - 阿拉伯呋喃糖-(1]→[5)-α - L - 阿拉伯呋喃糖-(1]→的重复单元组成，在C - 3位有分支：分支1为α - L - 阿拉伯呋喃糖-(1→，分支2为α - L - 阿拉伯呋喃糖-(1→4)-。此外，该结构还包括β - D - 葡萄糖-(1→[4)-β - D - 半乳糖-(1]→5)-α - L - 阿拉伯呋喃糖-(1→。在癌症和正常细胞系上进行的体外实验表明，纯化后的多糖对肺癌和肝癌细胞具有显著的促凋亡作用，而在125至1000μg/mL的剂量浓度范围内对正常组织细胞的生长没有影响。在1000μg/mL CVP - 2时，对A549细胞活力的抑制率为49.63%，对16HBE细胞为2.6%，对HepG2细胞为28.56%，对LX - 2细胞为12.64%。与无药组相比，A549、16HBE、HepG2和LX - 2细胞的死亡率分别增加了37.13%、4.15%、26.00%和4.41%。发现CVP - 2可抑制细胞周期的G1/S期。CVP - 2的潜在抗癌机制涉及癌细胞中Bcl - 2/Bax蛋白的减少，对正常细胞的生长无显著影响。因此，CVP - 2作为一种广谱抗肿瘤剂和膳食补充剂具有很大的前景。
