Chen Ping, Tan Xiao, Xiao LiuYue, Gong Zan, Qin Xiangxiang, Nie Jing, Zhu Hua, Zhong Shian
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Guangxi University of Chinese Medicine, Nanning 530200, China.
Int J Biol Macromol. 2025 Feb;290:138928. doi: 10.1016/j.ijbiomac.2024.138928. Epub 2024 Dec 17.
In this study, a water-soluble, homogeneous polysaccharide, GDPs-1, was isolated from Gynura divaricata and purified. It was subsequently subjected to structural characterization and biological activity assessment. Structural characterization revealed that GDPs-1 was mainly composed of glucose, galactose, and arabinose, with a molecular weight of 55.1 kDa. Its main structural backbone was →[4)-α-D-Glcp-(1] → 4)-α-D-Glcp-(1 → 4)-α-D-Glcp-(1 → [4)-β-D-Galp-(1]→, with two additional branched structures. In vitro experiments demonstrated that GDPs-1 induced cancer cell apoptosis, caused cell cycle blockade, increased the Bax/Bcl-2 protein ratio, attenuated epithelial-mesenchymal transition, and activated caspase-3 protein in carcinoma cells, thereby exerting antitumor effects on tumor cells without affecting the growth of normal cells. Therefore, GDPs-1 may be further explored as a novel medicine to treat lung and liver cancer.
在本研究中，从白凤菜中分离并纯化出一种水溶性、均一的多糖GDPs-1。随后对其进行了结构表征和生物活性评估。结构表征显示，GDPs-1主要由葡萄糖、半乳糖和阿拉伯糖组成，分子量为55.1 kDa。其主要结构主链为→[4)-α-D-葡萄糖-(1]→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→[4)-β-D-半乳糖-(1]→，还有两个额外的分支结构。体外实验表明，GDPs-1可诱导癌细胞凋亡，导致细胞周期阻滞，增加Bax/Bcl-2蛋白比值，减弱上皮-间质转化，并激活癌细胞中的caspase-3蛋白，从而对肿瘤细胞发挥抗肿瘤作用，而不影响正常细胞的生长。因此，GDPs-1有望作为一种治疗肺癌和肝癌的新型药物进一步研究。
