Hu Dayan, Jian Jieliang, Zhang Jinpeng, Xu Xiaojun, Wang Shu, Gong Cuiping, Zhang Yuanqin, Zhu Pengcan, Gu Zhimin, Guan Wenzhi
Huzhou Key Laboratory of Innovation and Application of Agricultural Germplasm Resources, Huzhou Academy of Agricultural Sciences, Huzhou, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Mol Biosci. 2024 Dec 11;11:1499220. doi: 10.3389/fmolb.2024.1499220. eCollection 2024.
Largemouth bass is an economically important farmed freshwater fish species that has delicious meat, no intermuscular thorns, and rapid growth rates. However, the molecular regulatory mechanisms underlying the different growth and developmental stages of this fish have not been reported.
In this study, we performed histological and transcriptomic analyses on the brain and dorsal muscles of largemouth bass at different growth periods. The brain and muscle tissue were dehydrated, embedded, sliced and stained with hematoxylin-eosin. Images were captured under a microscope and acquired using a microphotographic system. Differential expression between groups was analyzed using DESeq2. GO functional analysis and KEGG pathway analysis were then performed for differentially expressed genes. RT-qPCR validates the reliability of transcriptome sequencing data.
Smaller fish had more new muscle fiber numbers and wider intermuscular spaces compared to big specimens. Axons and nerve fibers were more pronounced in the telencephalons of big fish than in small fish. A total of 19,225 differentially expressed genes (DEGs) were detected in the muscle tissue, among which 7,724 were upregulated and 11,501 were downregulated, while a total of 5,373 DEGs were detected in the brain, among which 2,923 were upregulated and 2,450 were downregulated. GO and KEGG enrichment analyses indicated that nucleic acid binding, cytoskeletal motor activity, DNA binding, circadian rhythm, glycolysis/gluconeogenesis, and osteoclast differentiation were related to brain development while binding, cytoskeletal protein binding, biological processes, c-type lectin receptors, mitogen-activated protein kinase (MAPK) signaling pathways, and osteoclast differentiation were related to muscle growth. , , , , and genes were mainly involved in the growth and development of largemouth bass.
These results provide novel perspectives for deepening our understanding of the mechanisms underlying the growth and development and performing genetic selection in largemouth bass.
大口黑鲈是一种具有重要经济价值的养殖淡水鱼类，其肉质鲜美，无肌间刺，生长速度快。然而，关于该鱼类不同生长发育阶段的分子调控机制尚未见报道。
在本研究中，我们对不同生长时期大口黑鲈的脑和背肌进行了组织学和转录组分析。脑和肌肉组织经脱水、包埋、切片后进行苏木精-伊红染色。在显微镜下拍摄图像并使用显微摄影系统采集。使用DESeq2分析组间差异表达。然后对差异表达基因进行GO功能分析和KEGG通路分析。RT-qPCR验证转录组测序数据的可靠性。
与大鱼相比，小鱼的新肌纤维数量更多，肌间间隙更宽。大鱼端脑的轴突和神经纤维比小鱼更明显。在肌肉组织中共检测到19225个差异表达基因（DEGs），其中7724个上调，11501个下调；在脑中总共检测到5373个DEGs，其中2923个上调，2450个下调。GO和KEGG富集分析表明，核酸结合、细胞骨架运动活性、DNA结合、昼夜节律、糖酵解/糖异生和破骨细胞分化与脑发育相关，而结合、细胞骨架蛋白结合、生物过程、c型凝集素受体、丝裂原活化蛋白激酶（MAPK）信号通路和破骨细胞分化与肌肉生长相关。 、 、 、 、 和 基因主要参与大口黑鲈的生长发育。
这些结果为深化我们对大口黑鲈生长发育机制的理解以及进行遗传选择提供了新的视角。
