Kazerooni Elham Ahmed, Maharachchikumbura Sajeewa S N, Al-Sadi Abdullah Mohammed, Kang Sang-Mo, Yun Byung-Wook, Lee In-Jung
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, China.
J Fungi (Basel). 2021 Jun 10;7(6):472. doi: 10.3390/jof7060472.
The aim of this study was to assess the ability of , to augment plant growth and suppress gray mold and leaf spot in pepper plants. Morphological modifications in fungal pathogen hyphae that expanded toward the PGPR colonies were detected via scanning electron microscope. Furthermore, preliminary screening showed that PGPR could produce various hydrolytic enzymes in its media. Treatments with suppressed Botrytis gray mold and Alternaria leaf spot diseases on pepper caused by and , respectively. The PGPR strain modulated plant physio-biochemical processes. The inoculation of pepper with PGPR decreased protein, amino acid, antioxidant, hydrogen peroxide, lipid peroxidation, and abscisic acid levels but increased salicylic acid and sugar levels compared to those of uninoculated plants, indicating a mitigation of the adverse effects of biotic stress. Moreover, gene expression studies confirmed physio-biochemical findings. PGPR inoculation led to increased expression of the CaXTH genes and decreased expression of CaAMP1, CaPR1, CaDEF1, CaWRKY2, CaBI-1, CaASRF1, CaSBP11, and CaBiP genes. Considering its beneficial effects, the inoculation of can be proposed as an eco-friendly alternative to synthetic chemical fungicides.
本研究的目的是评估[具体内容缺失]促进辣椒植株生长以及抑制辣椒灰霉病和叶斑病的能力。通过扫描电子显微镜检测到向植物根际促生细菌（PGPR）菌落扩展的真菌病原体菌丝的形态学变化。此外，初步筛选表明PGPR能够在其培养基中产生多种水解酶。[具体处理方式缺失]处理分别抑制了由[具体病原菌1]和[具体病原菌2]引起的辣椒灰霉病和链格孢叶斑病。PGPR菌株调节了植物的生理生化过程。与未接种的植株相比，用PGPR接种辣椒降低了蛋白质、氨基酸、抗氧化剂、过氧化氢、脂质过氧化和脱落酸水平，但提高了水杨酸和糖水平，表明减轻了生物胁迫的不利影响。此外，基因表达研究证实了生理生化研究结果。PGPR接种导致CaXTH基因表达增加，而CaAMP1、CaPR1、CaDEF1、CaWRKY2、CaBI - 1、CaASRF1、CaSBP11和CaBiP基因表达降低。考虑到其有益效果，[具体PGPR种类缺失]接种可被提议作为合成化学杀菌剂的一种生态友好型替代方法。
