Konstantinou Lazaros, Varda Eleni, Apostolou Theofylaktos, Loizou Konstantinos, Dougiakis Lazaros, Inglezakis Antonios, Hadjilouka Agni
EMBIO Diagnostics Ltd., Athalassas, 2018 Nicosia, Cyprus.
Department of Life Sciences, School of Sciences, European University of Cyprus, 2404 Nicosia, Cyprus.
Biosensors (Basel). 2024 Nov 29;14(12):582. doi: 10.3390/bios14120582.
The prevalence of foodborne diseases is continuously increasing, causing numerous hospitalizations and deaths, as well as money loss in the agri-food sector and food supply chain worldwide. The standard analyses currently used for bacteria detection have significant limitations with the most important being their long procedural time that can be crucial for foodborne outbreaks. In this study, a biosensor system able to perform robust and accurate detection of spp. in meat products was developed. To achieve this, a portable device developed by EMBIO Diagnostics called B.EL.D (Bio Electric Diagnostics) and cell-based biosensor technology (BERA) were used. Results indicated that the new method could detect the pathogen within 24 h after a 3-min analysis and discriminate samples with and without with high accuracy. Achieving an accuracy of 86.1% and a detection limit (LOD) of 1 log CFU g, this innovative technology enables rapid and sensitive identification of spp. in meat and meat products, making it an excellent tool for pathogen screening.
食源性疾病的患病率持续上升，导致大量住院和死亡，以及全球农业食品部门和食品供应链的经济损失。目前用于细菌检测的标准分析方法存在重大局限性，其中最重要的是其漫长的检测过程，这对于食源性疾病暴发可能至关重要。在本研究中，开发了一种能够对肉类产品中的 菌进行可靠且准确检测的生物传感器系统。为此，使用了EMBIO诊断公司开发的名为B.EL.D（生物电诊断）的便携式设备和基于细胞的生物传感器技术（BERA）。结果表明，新方法在3分钟分析后24小时内即可检测到病原体，并且能够高精度地区分有无 菌的样本。该创新技术的准确率达到86.1%，检测限（LOD）为1 log CFU/g，能够快速、灵敏地鉴定肉类和肉类产品中的 菌，使其成为病原体筛查的优秀工具。
