Hao Xin, Zhang Yiyan, Zhang Hui, Yang Gang, Liu Zhou, Lv Huiwei, Zhou Xiaomei
College of Food Science and Engineering, Boda College of Jilin Normal University, Siping 136000, China.
College of International Education and Exchange, Jilin Agricultural University, Changchun 130118, China.
Curr Issues Mol Biol. 2024 Dec 15;46(12):14154-14167. doi: 10.3390/cimb46120847.
As a globally significant economic crop, the seed size of soybean ( [L.] Merr.) is jointly regulated by internal genetic factors and external environmental signals. This study discovered that the GLN family proteins in soybean are similar to the KIX-PPD-MYC transcriptional repressor complex in Arabidopsis, potentially influencing seed size by regulating the expression of the downstream gene . Additionally, β-1,3-glucanase (βGlu) plays a crucial role in antifungal activity, cell composition, flower development, pollen development, abiotic resistance, seed germination, and maturation in soybean. Through a detailed analysis of the structure, chromosomal localization, phylogenetic relationships, and expression situations in different tissues at different stages of the soybean gene family members, this research certifies a theoretical foundation for subsequent research on the biological functions of genes in soybean. This research incorporated a comprehensive genomic identification and expression analysis of the gene family in soybean. The results indicate that the 109 soybean genes are unevenly distributed across soybean chromosomes and exhibit diverse expression patterns in different tissues, suggesting they may have distinct functions in soybean morphogenesis. GO enrichment analysis shows that the gene family may participate in a variety of biological activities, cellular components, and molecular biological processes, particularly in catalytic activity, cellular components, and metabolic processes. These findings provide important information for comprehending the role of the gene family in soybean and offer potential targets for molecular breeding of soybean.
作为一种具有全球重要意义的经济作物，大豆（[L.] Merr.）的种子大小受内部遗传因素和外部环境信号共同调控。本研究发现，大豆中的GLN家族蛋白与拟南芥中的KIX-PPD-MYC转录抑制复合物相似，可能通过调节下游基因的表达来影响种子大小。此外，β-1,3-葡聚糖酶（βGlu）在大豆的抗真菌活性、细胞组成、花发育、花粉发育、非生物抗性、种子萌发和成熟中起关键作用。通过对大豆基因家族成员的结构、染色体定位、系统发育关系以及不同组织在不同阶段的表达情况进行详细分析，本研究为后续大豆基因生物学功能的研究奠定了理论基础。本研究对大豆基因家族进行了全面的基因组鉴定和表达分析。结果表明，109个大豆基因在大豆染色体上分布不均，在不同组织中表现出多样的表达模式，表明它们可能在大豆形态发生中具有不同的功能。GO富集分析表明，该基因家族可能参与多种生物活性、细胞成分和分子生物学过程，特别是催化活性、细胞成分和代谢过程。这些发现为理解该基因家族在大豆中的作用提供了重要信息，并为大豆分子育种提供了潜在靶点。
