Schultz Jonathan S, Okoli Mary, Lee Scott, Leonard Colleen M, Sayre Dean, Heilig Charles M, Uhomoibhi Perpetua, Ogunniyi Abiodun, Ndodo Nnaemeka, Mba Nwando, Abubakar Ado G, Akinmulero Oluwaseun, Dawurung Ayuba B, Okoye McPaul, Iriemenam Nnaemeka C, Plucinski Mateusz, Steinhardt Laura, Rogier Eric, Ihekweazu Chickwe
Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Sci Rep. 2024 Dec 28;14(1):30658. doi: 10.1038/s41598-024-74236-4.
Characterization of serological responses to Plasmodium falciparum (Pf) is of interest to understand disease burden and transmission dynamics; however, their interpretation is challenging. Dried blood spots from 30,815 participants aged 6 months to 15 years from the 2018 Nigeria HIV/AIDS Indicator and Impact Survey were analyzed by multiplex bead-based assay to measure immunoglobulin G (IgG) to Pf-stage-specific MSP-1, AMA-1, GLURPR0, LSA-1, and CSP. These IgG levels were analyzed by principal component analysis (PCA). PC1 and PC2 scores explained 41% and 17% of the total variance, respectively. PC1 unit vectors represented seropositivity. PC2 unit vectors for blood-stage antigens were in opposite directions to liver-stage and sporozoite antigens. PC2 scores were correlated with MSP-1 positively (R = 0.52, P < 0.001) and CSP negatively (R=-0.65, P < 0.001) and may help identify areas with prior exposure but higher risk for increased infections or epidemics. PCA of Pf serology can provide summary scores to possibly inform future programmatic interventions.
了解恶性疟原虫（Pf）的血清学反应特征对于理解疾病负担和传播动态具有重要意义；然而，对其进行解读具有挑战性。通过基于多重微珠的检测方法，对来自2018年尼日利亚艾滋病毒/艾滋病指标与影响调查的30815名年龄在6个月至15岁之间参与者的干血斑进行分析，以测量针对Pf阶段特异性MSP-1、AMA-1、GLURPR0、LSA-1和CSP的免疫球蛋白G（IgG）。通过主成分分析（PCA）对这些IgG水平进行分析。PC1和PC2得分分别解释了总方差的41%和17%。PC1单位向量代表血清阳性。血液阶段抗原的PC2单位向量与肝脏阶段和子孢子抗原的方向相反。PC2得分与MSP-1呈正相关（R = 0.52，P < 0.001），与CSP呈负相关（R = -0.65，P < 0.001），可能有助于识别既往有暴露但感染增加或流行风险较高的地区。Pf血清学的PCA可以提供汇总分数，可能为未来的规划干预提供参考。
