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解析24-表油菜素内酯对热胁迫条件下生长的小麦(Triticum aestivum L.)光合动力学及荧光参数的改善作用

Unravelling the photosynthetic dynamics and fluorescence parameters under ameliorative effects of 24-epibrassinolide in wheat (Triticum aestivum L.) grown under heat stress regime.

作者信息

Jat Manju, Ray Madhurya, Ahmad Md Afjal, Prakash Pravin

机构信息

Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University, BHU Varanasi, 221005, Uttar Pradesh, India.

出版信息

Sci Rep. 2024 Dec 28;14(1):30745. doi: 10.1038/s41598-024-79676-6.

Abstract

An experiment was performed at the Banaras Hindu University, India to study the effect of terminal heat stress on photosynthetic dynamics and fluorescence parameters of wheat genotypes and ameliorative effects of epibrassinolide by taking two genotypes with four concentrations as foliar spray at two growth stages of wheat. The highest values were observed in plots foliar sprayed with 1.0 µM 24-epibrassinolide (T1) under normal conditions (D1) where the genotype Sonalika (V1) performed significantly well w.r.t. the parameters viz. steady-state fluorescence (Fs) 116.22, quantum efficiency of PSII 0.59, maximum fluorescence (Fm) 776.5, normalized stress detection ratio (Fv/Fo) 4.47, maximum potential quantum efficiency of PSII (Fv/Fm) 0.82.Whereas under heat stress condition (late sown D2), there was significant reduction in these parameters in both the genotypes which was improved by the application of epibrassinolide suggesting its potential role in improving the photoinhibition process by raising the efficiency of PSII. Overall, the calibrated application of 24-epibrassinolide was found to be a potent growth regulator involved in the positive modulation of heat stress tolerance in wheat, coupled with improved photosynthetic efficiency in treated plots as compared to control.

摘要

在印度贝拿勒斯印度教大学进行了一项实验,以研究在小麦的两个生长阶段,终末热胁迫对小麦基因型光合动力学和荧光参数的影响,以及表油菜素内酯的改善作用。实验选取了两个基因型,以四种浓度进行叶面喷施。在正常条件下(D1),叶面喷施1.0µM 24-表油菜素内酯(T1)的地块中观察到最高值,其中基因型Sonalika(V1)在稳态荧光(Fs)116.22、PSII量子效率0.59、最大荧光(Fm)776.5、归一化胁迫检测率(Fv/Fo)4.47、PSII最大潜在量子效率(Fv/Fm)0.82等参数方面表现显著良好。而在热胁迫条件下(晚播D2),两个基因型的这些参数均显著降低,表油菜素内酯的施用改善了这些参数,表明其通过提高PSII效率在改善光抑制过程中具有潜在作用。总体而言,发现24-表油菜素内酯的校准施用是一种有效的生长调节剂,参与了小麦热胁迫耐受性的正向调节,与对照相比,处理地块的光合效率有所提高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba03/11680823/42c0e2c99e51/41598_2024_79676_Fig1_HTML.jpg

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