Yang Xiliang, Xia Lingyun, Chen Yongji, Jiang Lei, Zheng Tianxia, Bai Yuhong
Department of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, People's Republic of China.
Department of Oral, College of Stomatology, North China University of Science and Technology, Tangshan City, Hebei Province, People's Republic of China.
Aust Endod J. 2025 Apr;51(1):124-132. doi: 10.1111/aej.12916. Epub 2024 Dec 27.
This study evaluated the cytotoxicity and biocompatibility of a new strontium silicate-based root canal sealer (C-Root SP), in comparison with those of iRoot SP and AH plus. The sealer extract was diluted to the concentrations of 100%, 75%, 50%, and 25%. L929 cells were cultured for 24 h, and the absorbance value was determined. Two-way analysis of variance (two-way ANOVA) and sealers were implanted in the tibia of Sprague-Dawley (SD) rats, At 2, 6, and 12 weeks, rats were euthanised. The tissue reaction was evaluated by HE staining. The least significant difference (LSD) t-test and Kruskal-Wallis test were used. The cytotoxicity of C-Root SP and iRoot SP was found to be less than that of AH plus. At 12 weeks, new bone formation was induced around C-Root SP and iRoot SP sealer, but minimal evidence of bone formation was found in AH plus. C-Root SP has low cytotoxicity and superior biocompatibility.
本研究评估了一种新型硅酸锶基根管封闭剂（C-Root SP）与iRoot SP和AH plus相比的细胞毒性和生物相容性。将封闭剂提取物稀释至100%、75%、50%和25%的浓度。培养L929细胞24小时，并测定吸光度值。采用双向方差分析（two-way ANOVA），将封闭剂植入Sprague-Dawley（SD）大鼠的胫骨中。在第2、6和12周时，对大鼠实施安乐死。通过苏木精-伊红（HE）染色评估组织反应。使用最小显著差（LSD）t检验和Kruskal-Wallis检验。发现C-Root SP和iRoot SP的细胞毒性低于AH plus。在第12周时，C-Root SP和iRoot SP封闭剂周围诱导了新骨形成，但在AH plus中发现的骨形成证据极少。C-Root SP具有低细胞毒性和优异的生物相容性。
