Abdollahi Amir Ardalan, Seyyedi Seyyed Amir, Lotfollahi Lida, Javan Navid Yekadast
Department of Endodontics, Dental School, Urmia University of Medical Sciences, Urmia, Iran.
Department of Oral and Maxillofacial Medicine, Dental School, Urmia University of Medical Sciences, Urmia, Iran.
Dent Res J (Isfahan). 2024 Nov 26;21:62. doi: 10.4103/drj.drj_260_24. eCollection 2024.
This study compared the antibacterial effects of 940 nm diode laser and sodium hypochlorite and chlorhexidine irrigations on in human permanent single-rooted teeth.
In this study, 65 extracted human single-rooted teeth were prepared using the crown-down method using rotary files. The root canals were irrigated with 5.25% sodium hypochlorite, 17% ethylenediaminetetraacetic acid, and normal saline solution. After placing the roots in microtubules, they were transferred into an autoclave. The teeth were randomly divided into four groups ( = 15): laser, sodium hypochlorite, chlorhexidine, and saline. Three teeth were assigned to the positive control group and two to the negative control group. The root canals were sampled, and the colony counts were determined 24 h later. Then, antibacterial agents were applied to the canals, and immediately after, the root canals were sampled, and the colony counts were determined 24 h later. The data were analyzed using Kruskal-Wallis and Mann-Whitney -tests using the SPSS software version 26. The significance level was defined at < 0.05.
The results showed that sodium hypochlorite, chlorhexidine, laser, and normal saline significantly reduced bacterial colony counts, confirming their antimicrobial effects ( < 0.001). Sodium hypochlorite and chlorhexidine showed the highest antimicrobial effects, with no significant differences between the sodium hypochlorite and chlorhexidine groups ( = 0.512); however, there were significant differences between the other groups ( < 0.001).
According to the results, 940 nm diode laser beams significantly reduced . counts and could be used as a new, effective, and complementary treatment in disinfecting the root canal.
本研究比较了940纳米二极管激光与次氯酸钠和氯己定冲洗液对人恒牙单根牙的抗菌效果。
在本研究中，使用旋转锉采用冠向下法对65颗拔除的人单根牙进行预备。根管用5.25%次氯酸钠、17%乙二胺四乙酸和生理盐水冲洗。将牙根放入小管后，转移至高压灭菌器中。牙齿随机分为四组（每组n = 15）：激光组、次氯酸钠组、氯己定组和生理盐水组。三颗牙齿分配到阳性对照组，两颗牙齿分配到阴性对照组。对根管进行取样，并在24小时后测定菌落计数。然后，将抗菌剂应用于根管，之后立即对根管进行取样，并在24小时后测定菌落计数。使用SPSS 26版软件进行Kruskal-Wallis检验和Mann-Whitney U检验分析数据。显著性水平定义为P < 0.05。
结果表明，次氯酸钠、氯己定、激光和生理盐水均显著降低了细菌菌落计数，证实了它们的抗菌作用（P < 0.001）。次氯酸钠和氯己定显示出最高的抗菌效果，次氯酸钠组和氯己定组之间无显著差异（P = 0.512）；然而，其他组之间存在显著差异（P < 0.001）。
根据结果，940纳米二极管激光束显著降低了菌落计数，可作为根管消毒的一种新的、有效的辅助治疗方法。
