Li Sihan, Chu Liangyu, Zhang Yancheng, Yu Yaxuan, Wang Guoqing
College of Medicine and Biological Information Engineering, Northeastern University, Shengyang, China.
College of Life and Health Sciences, Northeastern University, Shengyang, China.
Front Vet Sci. 2024 Dec 13;11:1501632. doi: 10.3389/fvets.2024.1501632. eCollection 2024.
Canine calicivirus (CaCV) belongs to the family, which invades multiple host species. Notably, there are distinct serological and genetic differences between CaCV and other caliciviruses. However, the genome data for only 13 strains of CaCV have been recorded. Moreover, there have been no reports on the CaCV genome in China. To understand the genetic characteristics of CaCV in China, this study tested CaCV in 52 canine nasal swab samples by RT-PCR and finally determined that two samples were positive for this virus. The complete genome sequences of both CaCV strains were obtained through sequencing, with a genomic length of 8,453 bp. The genomic sequences of the two Chinese CaCV strains presented 83.6% nucleotide similarity with each other but 71.6%-90.2% nucleotide similarity with previously reported CaCV strains, indicating that these two CaCV strains were genetically divergent. The subsequent sequence analysis indicated that these two strains possessed unique nucleotide sites in their genomic sequences. Phylogenetic analysis revealed that the Chinese CaCV strains belonged to different evolutionary branches. Overall, the present study provides new knowledge on the prevalence and genetic diversity of CaCV in China.
犬杯状病毒（CaCV）属于该科，可侵袭多种宿主物种。值得注意的是，CaCV与其他杯状病毒之间存在明显的血清学和基因差异。然而，仅记录了13株CaCV的基因组数据。此外，中国尚未有关于CaCV基因组的报道。为了解中国CaCV的遗传特征，本研究通过RT-PCR对52份犬鼻拭子样本进行了CaCV检测，最终确定两份样本对该病毒呈阳性。通过测序获得了两个CaCV毒株的完整基因组序列，基因组长度为8453 bp。这两个中国CaCV毒株的基因组序列彼此具有83.6%的核苷酸相似性，但与先前报道的CaCV毒株具有71.6%-90.2%的核苷酸相似性，表明这两个CaCV毒株在基因上存在差异。随后的序列分析表明，这两个毒株在其基因组序列中具有独特的核苷酸位点。系统发育分析显示，中国的CaCV毒株属于不同的进化分支。总体而言，本研究为中国CaCV的流行情况和遗传多样性提供了新知识。
