文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

一种基于壳聚糖/脱细胞基质并携带间充质干细胞的神经移植物，用于促进周围神经修复。

A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair.

作者信息

Zhang Zhifa, Li Molin, Cheng Gang, Wang Peng, Zhou Chunhui, Liu Yang, Duan Xiaofeng, Wang Jing, Xie Fang, Zhu Yaqiong, Zhang Jianning

机构信息

Departments of Neurosurgery, The First Center of Chinese, PLA General Hospital, Beijing, China.

Departments of Ultrasound, Tianjin Medical University Cancer Institute & Hospital, Tianjin, China.

出版信息

Stem Cell Res Ther. 2024 Dec 31;15(1):503. doi: 10.1186/s13287-024-04093-5.

DOI:10.1186/s13287-024-04093-5
PMID:39736729
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11687163/
Abstract

BACKGROUND: Treatment of peripheral nerve defects is a major concern in regenerative medicine. This study therefore aimed to explore the efficacy of a neural graft constructed using adipose mesenchymal stem cells (ADSC), acellular microtissues (MTs), and chitosan in the treatment of peripheral nerve defects. METHODS: Stem cell therapy with acellular MTs provided a suitable microenvironment for axonal regeneration, and compensated for the lack of repair cells in the neural ducts of male 8-week-old Sprague Dawley rats. RESULTS: In vitro, acellular MTs retained the intrinsic extracellular matrix and improved the narrow microstructure of acellular nerves, thereby enhancing cell functionality. In vivo neuroelectrophysiological studies, gait analysis, and sciatic nerve histology demonstrated the regenerative effects of active acellular MT. The Chitosan + Acellular-MT + ADSC group exhibited superior myelin sheath quality and improved neurological and motor function recovery. CONCLUSIONS: Active acellular-MTs precellularized with ADSC hold promise as a safe and effective clinical treatment method for peripheral nerve defects.

摘要

背景：周围神经缺损的治疗是再生医学中的一个主要关注点。因此，本研究旨在探讨使用脂肪间充质干细胞（ADSC）、脱细胞微组织（MTs）和壳聚糖构建的神经移植物在治疗周围神经缺损中的疗效。 方法：脱细胞MTs的干细胞疗法为轴突再生提供了合适的微环境，并弥补了8周龄雄性Sprague Dawley大鼠神经导管中修复细胞的不足。 结果：在体外，脱细胞MTs保留了内在的细胞外基质并改善了脱细胞神经的狭窄微观结构，从而增强了细胞功能。体内神经电生理研究、步态分析和坐骨神经组织学证明了活性脱细胞MT的再生作用。壳聚糖+脱细胞-MT+ADSC组表现出 superior 髓鞘质量，并改善了神经和运动功能恢复。 结论：用ADSC预细胞化的活性脱细胞-MTs有望成为一种安全有效的周围神经缺损临床治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/5aaa4d971db2/13287_2024_4093_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/0237aebd54d7/13287_2024_4093_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/29976c28c700/13287_2024_4093_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/490ae48674f3/13287_2024_4093_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/8e7735fee065/13287_2024_4093_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/1c86e3f73f1c/13287_2024_4093_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/a46c44caf872/13287_2024_4093_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/5aaa4d971db2/13287_2024_4093_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/0237aebd54d7/13287_2024_4093_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/29976c28c700/13287_2024_4093_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/490ae48674f3/13287_2024_4093_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/8e7735fee065/13287_2024_4093_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/1c86e3f73f1c/13287_2024_4093_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/a46c44caf872/13287_2024_4093_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/770b/11687163/5aaa4d971db2/13287_2024_4093_Fig7_HTML.jpg

相似文献

[1]
A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair.

Stem Cell Res Ther. 2024-12-31

[2]
Biological characteristics of tissue engineered-nerve grafts enhancing peripheral nerve regeneration.

Stem Cell Res Ther. 2024-7-18

[3]
Recellularized nerve allografts with differentiated mesenchymal stem cells promote peripheral nerve regeneration.

Neurosci Lett. 2012-3-3

[4]
Harnessing three-dimensional porous chitosan microsphere embedded with adipose-derived stem cells to promote nerve regeneration.

Stem Cell Res Ther. 2024-6-1

[5]
Transplantation of adipose-derived stem cells for peripheral nerve repair.

Int J Mol Med. 2011-6-17

[6]
Schwann cells and mesenchymal stem cells in laminin- or fibronectin-aligned matrices and regeneration across a critical size defect of 15 mm in the rat sciatic nerve.

J Neurosurg Spine. 2018-1

[7]
Mesenchymal Stem Cells Expressing Baculovirus-Engineered Brain-Derived Neurotrophic Factor Improve Peripheral Nerve Regeneration in a Rat Model.

Tissue Eng Regen Med. 2025-4

[8]
A nerve graft constructed with xenogeneic acellular nerve matrix and autologous adipose-derived mesenchymal stem cells.

Biomaterials. 2010-4-8

[9]
Transplantation of bone marrow stromal stem cells overexpressing tropomyosin receptor kinase A for peripheral nerve repair.

Cytotherapy. 2017-8

[10]
Regeneration of Rat Sciatic Nerve Using PLGA Conduit Containing Rat ADSCs with Controlled Release of BDNF and Gold Nanoparticles.

J Mol Neurosci. 2021-4

引用本文的文献

[1]
Mesenchymal stem cell-based therapy for peripheral nerve injuries: A promise or reality?

World J Stem Cells. 2025-6-26

[2]
Overcoming acquired immunotherapy resistance in non-small cell lung cancer using ginsenoside Rb1-loaded, peptide-enhanced exosome delivery systems.

J Nanobiotechnology. 2025-6-13

本文引用的文献

[1]
Bioactive Peptide Hydrogel Scaffold with High Fluidity, Thermosensitivity, and Neurotropism in 3D Spatial Structure for Promoted Repair of Spinal Cord Injury.

Small. 2025-1

[2]
Scaffold-based tissue engineering strategies for urethral repair and reconstruction.

Biofabrication. 2024-11-1

[3]
Chiral Hydrogel Nerve Conduit Boosts Peripheral Nerve Regeneration via Regulation of Schwann Cell Reprogramming.

ACS Nano. 2024-10-15

[4]
Skin organoid transplantation promotes tissue repair with scarless in frostbite.

Protein Cell. 2025-4-18

[5]
Extracellular vesicle therapy in neurological disorders.

J Biomed Sci. 2024-8-25

[6]
Multilevel neurium-mimetic individualized graft via additive manufacturing for efficient tissue repair.

Nat Commun. 2024-7-31

[7]
Engineered Regenerative Isolated Peripheral Nerve Interface for Targeted Reinnervation.

Adv Mater. 2024-11

[8]
Biological characteristics of tissue engineered-nerve grafts enhancing peripheral nerve regeneration.

Stem Cell Res Ther. 2024-7-18

[9]
Piezoelectrically-activated antibacterial catheter for prevention of urinary tract infections in an on-demand manner.

Mater Today Bio. 2024-5-11

[10]
Ultrasound-Responsive Aligned Piezoelectric Nanofibers Derived Hydrogel Conduits for Peripheral Nerve Regeneration.

Adv Mater. 2024-7

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索