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锐齿沙蟹（十足目：沙蟹科）中17个新型多核苷酸微卫星标记的鉴定与特征分析

Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae).

作者信息

Song Ha Yeun, Choi Young Ji, Lee Dea-Sung, Choi Hyun Ki, Choi Kyung Min, Yu Kang-Yeol, Cho Namki, Lee Seungki

机构信息

Honam National Institute of Biological Resources, Mokpo, 58762, Republic of Korea.

National Marine Biodiversity Institute of Korea, Seochun, 33662, Republic of Korea.

出版信息

BMC Res Notes. 2024 Dec 30;17(1):396. doi: 10.1186/s13104-024-07049-3.

DOI:10.1186/s13104-024-07049-3
PMID:39736772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11684255/
Abstract

OBJECTIVE

The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing.

RESULTS

Out of the 63 loci examined, 26 were effectively amplified in a sample of 100 individuals. These 17 loci, comprising four tri-nucleotide and 13 tetra-nucleotide repeats, exhibited a range of 4 to 26 alleles per locus (with an average of 14.1 alleles) across 100 samples from three O. stimpsoni populations. The mean observed and expected heterozygosities were calculated to be 0.885 and 0.836, respectively. These 17 newly identified polymorphic microsatellite loci hold valuable utility for investigating the genetic structure and diversity of this protected marine species.

摘要

目的

由于幽灵蟹（Ocypode stimpsoni，十足目）种群数量下降，它在韩国被指定为受保护的海洋物种。在本研究中，我们通过下一代测序成功鉴定出17个幽灵蟹微卫星标记。

结果

在所检测的63个位点中，有26个位点在100个个体的样本中得到有效扩增。这17个位点，包括4个三核苷酸重复和13个四核苷酸重复，在来自三个幽灵蟹种群的100个样本中，每个位点的等位基因数在4到26个之间（平均为14.1个等位基因）。观察到的平均杂合度和预期杂合度分别计算为0.885和0.836。这17个新鉴定的多态性微卫星位点对于研究这种受保护海洋物种的遗传结构和多样性具有重要价值。

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