Ibraheim Hanaa Khaleel, Madhi Khadeeja S, Jasim Alyaa Sabti, Gharban Hasanain A J
Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basra, Iraq.
Department of Microbiology, College of Medicine, University of Basrah, Basra, Iraq.
Open Vet J. 2024 Nov;14(11):2980-2988. doi: 10.5455/OVJ.2024.v14.i11.26. Epub 2024 Nov 30.
In goats, acute and chronic respiratory infections are often characterized by a rapidly progressing clinical course with little opportunity to develop an effective antibiotic therapy.
This study aimed to identify spp. in pneumonic goats, assess its antibiotic susceptibility, and confirm the molecular phylogenetics of spp.
A total of 80 pneumonic goats were selected from the slaughterhouses located in Basra province (Iraq) from June to November 2023, and each animal was subjected to obtaining only one sample. The studied samples were included 30 nasal swabs obtained from the lived goats, while 30 lung samples in addition to 20 tracheal swabs were collected from slaughtered goats. All study samples were inoculated onto MacConkey agar and tested biochemically. Eleven types of antibiotics were served in the Kirby-Bauer disc diffusion method to identify the susceptibility of spp. Positive culture isolates were tested molecularly using the polymerase chain reaction (PCR) and then sequenced for phylogenetic analysis of study isolates.
The findings indicated that 35% of samples were positive to spp. including 30% in trachea, 33.33% in nasal swabs, and 40% in lungs. colonies appeared on MacConkey agar as bright pink mucoid texture; while on blood agar, they were large, glossy, mucoid, whitish-grey, spherical, and free of hemolysis. Biochemically, all isolates were exhibited a negative reactivity to motility, oxidase, indole, and methyl red, but positives to urease, citrate utilization, catalase, and Voges-Proskauer, acid and gas production. Antibiotic susceptibility testing revealed the high susceptibility of isolates to meropenem (71.43%), and intermediate susceptibility to ciprofloxacin (28.57%), but high resistance to imipenem (60.71%). Targeting the gene, PCR results confirmed all tested isolates as spp. Finally, phylogenetic analysis of 9 positive isolates demonstrated the identity of local isolates to (no = 4), (no = 3), (no = 1), and (no = 1).
Our study confirms the presence of , , and in pneumonic goats, highlighting the importance of molecular phylogeny in the detection of new species. However, furthermore studies are necessary to investigate various species/strains in goats and other domestic animals.
在山羊中，急性和慢性呼吸道感染通常表现为临床病程迅速进展，几乎没有机会开展有效的抗生素治疗。
本研究旨在鉴定患肺炎山羊体内的 菌属，评估其抗生素敏感性，并确认 菌属的分子系统发育情况。
2023年6月至11月，从伊拉克巴士拉省的屠宰场选取了80只患肺炎的山羊，每只动物仅采集一个样本。所研究的样本包括从存活山羊身上采集的30份鼻拭子，以及从屠宰山羊身上采集的30份肺样本和20份气管拭子。所有研究样本均接种于麦康凯琼脂培养基上，并进行生化测试。采用 Kirby-Bauer 纸片扩散法使用11种抗生素来鉴定 菌属的敏感性。对阳性培养分离株进行聚合酶链反应（PCR）分子检测，然后测序以对研究分离株进行系统发育分析。
研究结果表明，35%的样本对 菌属呈阳性，其中气管样本阳性率为30%，鼻拭子样本为33.33%，肺样本为40%。 菌落在麦康凯琼脂培养基上呈现亮粉色黏液质地；而在血琼脂培养基上，它们较大、有光泽、呈黏液状、灰白色、球形，且不溶血。生化检测显示，所有分离株对动力、氧化酶、吲哚和甲基红呈阴性反应，但对尿素酶、柠檬酸盐利用、过氧化氢酶和Voges-Proskauer试验呈阳性，能产酸产气。抗生素敏感性测试显示， 分离株对美罗培南高度敏感（71.43%），对环丙沙星中度敏感（28.57%），但对亚胺培南高度耐药（60.71%）。针对 基因进行PCR检测，结果证实所有测试分离株均为 菌属。最后，对9株阳性分离株的系统发育分析表明，本地 分离株与 （4株）、 （3株）、 （1株）和 （1株）相同。
我们的研究证实了患肺炎山羊体内存在 、 和 菌属，突出了分子系统发育在检测新 菌种中的重要性。然而，有必要进一步开展研究，以调查山羊和其他家畜中的各种 菌种/菌株。
