Tang Y, Li Y, Lin S, Wei W, Chen H
Institute of Medical Imaging, Guangzhou Panyu Central Hospital, Guangzhou, China.
Guangdong Cord Blood Bank, Guangzhou, China.
Bull Exp Biol Med. 2024 Dec;178(2):273-279. doi: 10.1007/s10517-025-06320-2. Epub 2025 Jan 6.
Cerebral palsy (CP) is the most common neuromuscular disorder in children with no effective therapeutic methods. To examine CP, a large variety of methods and animal models was developed, the most popular are the hypoxic-ischemic (HI) injury and/or LPS injection in mice. In the presented work, HI and LPS were applied on the postnatal day 9 to humanized immunodeficiency mouse pups, thereupon 3 behavioral tests were performed in 8 weeks later. Both HI and LPS caused significant behavioral deficits assessed in the Rotarod test. In gait dynamics and open-field tests, HI and LPS caused significant behavioral deficits reported by some parameters, and the effect of HI was more severe. Additionally, HI and LPS produced the different effects on gait dynamics of the fore and hind paws. Thus, both HI and LPS induced the behavioral disorders in mice, but HI was more suitable for the development of humanized immunodeficiency mouse model of CP.
脑瘫（CP）是儿童中最常见的神经肌肉疾病，目前尚无有效的治疗方法。为了研究脑瘫，人们开发了各种各样的方法和动物模型，其中最常用的是对小鼠进行缺氧缺血（HI）损伤和/或注射脂多糖（LPS）。在本研究中，在出生后第9天对人源化免疫缺陷小鼠幼崽施加HI和LPS，随后在8周后进行3项行为测试。在转棒试验中，HI和LPS均导致显著的行为缺陷。在步态动力学和旷场试验中，HI和LPS通过一些参数导致了显著的行为缺陷，且HI的影响更严重。此外，HI和LPS对前爪和后爪的步态动力学产生了不同的影响。因此，HI和LPS均诱导了小鼠的行为障碍，但HI更适合用于开发人源化免疫缺陷小鼠脑瘫模型。
